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Stem-cell technology is a major area within cell therapy that promises significant therapeutic outcome. The plasticity and self-renewal capabilities of stem cells make them valuable tools for potential application in regenerative medicine and tissue replacement following injury or disease. Different types of stem cells currently are used in research, preclinical and early clinical development, their potential therapeutic and diagnostic applications and current barriers to translating basic research into clinical therapies. Biomedical imaging is increasingly being used to monitor the fate of transplanted stem cells, including their survival, proliferation, differentiation and homing to targeted organs and tissues. Currently many researchers are utilized to track stem calls, the advantages and challenges, future implications in clinical applications.