Forensic experts may face challenging situations in the routine of human identifications, such as the lack of ante-mortem (AM) data; and cadaveric alterations, such as charred, mutilated and decomposed bodies, as well as skeletal remains. In this context, alternative pathways for human identification must be taken following scientific criteria. The present study reports a case of human identification combining the analysis of smile photographs and forensic anthropology exams. Skeletal remains of were referred for human identification in the local medico-legal institute. The anthropological profile of the victim, containing age, sex, stature and ancestry information pointed out a potential victim from a police list of missing persons. Relatives of the victims did not provide AM data; except for a single smile photograph form the victim’s personal belongings. Dental autopsy, previously guided by anthropological exams, culminated in positive identification. Similar to all the other pathways for human identification, the analysis of smile photographs presents advantages and limitations. However, when combined to other methods, such as forensic anthropology, smile photographs trend the reveal greater application.