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Introduction: Hypothermia is highly prevalent and a major contributor of neonatal morbidity and mortality, even in warmer tropical countries. Neonatal hypothermia is increasingly recognized as a risk factor for new-born survival in Ethiopia. It is crucial and effective to reduce perinatal and neonatal morbidity and mortality. The aim of the study was to assess the proportion of neonatal hypothermia and associated factors among new-borns at Gondar University Teaching and Referral Hospital, Northwest Ethiopia, 2014. Methods: Across sectional study was carried out from September to October 2014 at Gondar University teaching and referral Hospital. A total of 421 new-borns delivered in the hospital were included using systematic random sampling technique. Data were entered into Epi data version 3.1 and analyzed by Statistical package for social science version 20. P-value <0.05 was taken as statistically significant association.