alexa Saccharification

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Saccharification

Saccharification condition: Pretreated woody powders (200 g) were added to 800 ml of 20 mM citrate buffer (pH 5.0), mixed with 9 FPU/g of the dry substrate Trichoderma viride cellulase and 18 μl/g of the dry substrate Optimash BG enzyme, and incubated a 37°C at 120 rpm. Reaction mixtures were collected every 24 hours, boiled for 10 min, and centrifuged at 20,000 × g for 10 min at 4°C. The supernatant was filtered through a 0.2 μm filter (Merck Millipore, Billerica, MA, USA). Filtered samples were used as hydrolysates, and glucose concentrations in the solutions were measured by highperformance liquid chromatography (HPLC) performed under the following conditions: detector, Model RI-8020; columns, TSK-gel connected in that order; mobile phase, deionized water; flow rate, 0.5 ml/min; column oven, Model CO8020C (Tosoh) at 60°C. The total phenol content was quantified according to the Folin- Ciocalteau method. All experiments were performed in triplicate and standard deviation was calculated from obtained data.

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