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During the last few decades, extensive attention has been paid on the management of food alteration and contamination caused by spoilage organisms such as Pseudomonas aeruginosa and Pseudomonas fluorescens. The ability to metabolize a variety of diverse nutrients enables these bacteria to survive in a variety of habitats by developing biofilms. Biofilms are more resistant to antimicrobials making their elimination from food processing facilities a big challenge. However, understanding the mechanisms by which these microorganisms develop biofilm has remained elusive. In this background and because of their resistant phenotype, attention should be focused on better understanding how to eradicate completely Pseudomonas biofilm.