chromatography-2017-posters-accepted-abstracts

14 / 35

Page 65
conferenceseries.com
Volume 8, Issue 5 (Suppl) J Chromatogr Sep Tech, an open access journal
ISSN: 2157-7064 Chromatography 2017
August 07-09, 2017
August 07-09, 2017 | Rome, Italy
4th World Congress on
Chromatography
Residue analysis of amisulbrom in oriental melon (Cucumis melo L. var. makuwa) cultivated in plastic
house conditions: Dissipation kinetics, pre-harvest residue limits, and risk quotients assessment
Md Humayun Kabir1, Md Musfiqur Rahman1, A M Abd El-Aty2, Hyung Suk Chung1, Han Sol Lee1 and Jae-Han Shim1
1Chonnam National University, South Korea
2Konkuk University, South Korea
Amisulbrom residue levels in oriental melon were determined using liquid chromatography–ultraviolet detector (LC-
UVD). Real sample mass confirmation was performed with liquid chromatography-mass spectrometry (LC-MS/MS).
Samples were prepared using liquid-liquid extraction (LLE) and cleaned up with solid phase extraction (SPE) florisil (1 g, 6 cc).
The standard showed good instrument response linearity. Its determination coefficient R2=0.9999 and recovery ranged from
87.5% to 97.3%. The dissipation patterns of this pesticide were determined using samples from two different sites. Half-lives
were assessed at 7.0 d and 8.8 d for site 1 and site 2, respectively. A pre-harvest residue limit graph was also constructed from
the data. It indicated that if the residue levels were less than 0.55-0.59 mg/kg 3 d before harvest or less than 0.61-0.74 mg/kg 7
d before harvest, then they would be lower than the maximum residue levels. Risk assessments showed that the risk quotient
was 4.39-3.47% at 0 d and declined to 1.53-1.63% at 10 d. Therefore, the experimental data indicate that the amisulbrom dosage
recommended for oriental melon is unlikely to induce adverse health effects in Korean consumers.
Biography
Md Humayun Kabir completed his BSc (Hons) and MS degree in Chemistry from University of Dhaka, and working as Scientific Officer at Bangladesh Council of
Scientific and Industrial Research, Bangladesh. Currently, he is pursuing PhD under the supervision of Professor Shim Jae Han at Chonnam National University,
Republic of Korea. He is interested in food safety from harmful materials such as pesticides and antibiotics. He participated in many projects to develop the
analytical method for determining pesticides and antibiotics in several matrices such as vegetables and fruits.
Md Humayun Kabir et al., J Chromatogr Sep Tech 2017, 8:5(Suppl)
DOI: 10.4172/2157-7064-C1-032