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.com
Volume 8, Issue 5 (Suppl)
J Chromatogr Sep Tech, an open access journal
ISSN: 2157-7064
Chromatography 2017
August 07-09, 2017
August 07-09, 2017 | Rome, Italy
4
th
World Congress on
Chromatography
Application of a strategy based on metabolomics guided promoting blood circulation bioactivity
compounds screening of vinegar
Zhenli Liu
Institution of Basic Theory-China Academy of Chinese Medical Sciences, China
Statement of the Problem:
Rice vinegar (RV) and white vinegar (WV) as daily flavoring, have also used as accessory in
traditional Chinese medicine processing. As we know, the promoting blood circulation efficiency could be enhanced when
herbs processed by vinegar. Number of reports focused on health benefits derived by consumption of vinegar. However, few
concerned the blood circulation bioactivity.
Methodology & Theoretical Orientation:
In this paper, a metabolomics guided strategy was proposed to elaborate on the
chemical constituents’ variation of two kinds of vinegar. GC-MS coupled with multivariate statistical analysis were conducted
to analyze the chemical components in RV and WV and to discriminate them. The anti-platelet activities
in vitro
were
investigated by whole blood aggregometry platelet test. And the anticoagulant activities were monitored by the whole blood
viscosity, plasma viscosity, packed cell volume, prothrombin time, and four coagulation tests (PT, TT, APTT, FIB)
in vivo
.
Findings:
Constituents of RV andWV were globally characterized and 33 potential biomarkers were identified. The contents of
four potential alkaloid biomarkers increased with aging time prolonged in RV. RV and its alkaloids metabolites exhibited some
anti-platelet effects
in vitro
and anticoagulant activities
in vivo
. WV failed to exhibit promoting effects.
Conclusion & Significance:
Alkaloid metabolites were demonstrated to be the principal compounds contributing to
discrimination and it increased with aging time prolonged in RV. RV exhibited the blood circulation bioactivity. The alkaloids
of RV contributed to the blood circulation bioactivity.
Figure 1:
Description of the strategy
Biography
Zhenli Liu has her expertise in “The quality control of Chinese herbal medicine using analytical methods and in separating active ingredients from herbal medicine
using chemical separation methods”. She has published more than 100 journal articles in both Chinese and English.
zhenli_liu@sina.comZhenli Liu, J Chromatogr Sep Tech 2017, 8:5(Suppl)
DOI: 10.4172/2157-7064-C1-032