chromatography-2017-posters-accepted-abstracts

22 / 35

Page 74
Notes:
conferenceseries.com
Volume 8, Issue 5 (Suppl) J Chromatogr Sep Tech, an open access journal
ISSN: 2157-7064 Chromatography 2017
August 07-09, 2017
August 07-09, 2017 | Rome, Italy
4th World Congress on
Chromatography
Advantages of using a microfluidic-chip as sample treatment miniaturization for a subsequent
analysis by HPLC
María Ramos Payan
Instituto de Microelectrónica de Barcelona IMB-CNM (CSIC), Spain
Statement of the Problem: The most critical stage of the analytical process is the preparation of the sample requiring different
stages prior to analysis, long extraction times, large volumes of reagents, etc., with the objective of obtaining a good clean-
up for the analysis by instrumental techniques (as HPLC). Nowadays, one of the dominant trends in sample preparation is
miniaturization and automation. In this paper, we present the advantages presented by the use of microfluidic systems in this
field. These devices allow working in different configurations depending on the nature of the analyte to be extracted: either by
liquid phase micro-extraction or by electro membranes.
Methodology: Our group has demonstrated the possibility of on-line and off-line analysis by HPLC. Two pumps are used
to introduce the sample and the acceptor phase into the microfluidic device. The microfluidic device is fabricated using
two patterned plates of poly (methyl methacrylate), which are symmetrical. The channels are separated by a polypropylene
membrane. For off-line analysis, the acceptor outlet (extract) is collected and injected directly into a HPLC. For on-line
analysis, the acceptor outlet is connected to the HPLC.
Findings: This type of device provides high selectivity, clean-up, reduces sample volume and low consumption of reagents,
significantly reduces time of analysis and has demonstrated its ability to online coupling with HPLC. Additionally, the
microchip-devices are reusable (allow membrane exchange) and each membrane is stable during more than 10 consecutive
micro extractions.
Conclusion & Significance: The miniaturization and automatization of sample treatment procedures (on-chip) offer
multiple advantages compared with existing traditional techniques. It also, offers excellent clean-up either with biological or
environmental samples and significantly reduces the time of analysis from the sample collection till data obtaining.
Biography
María Ramos Payan has expertise in “Improving sample preparation techniques focused on microfluidic-chip devices as miniaturization”. The novelty of her
microfluidic devices offer more advantages than the existing methodologies. The devices work either using biological and environmental samples and can be
coupled on-line to HPLC or mass spectrometry. She has also demonstrated the applicability of microchip devices for diagnostic diseases as diabetes. She has
worked at different institutions (University of Seville, University of Huelva, University of Lund, University of Copenhagen and University of North Carolina, USA).
Currently, she works at Microelectronic National Center of Barcelona and Universitat Autonoma of Barcelona with the aim of implementing optical detection into
microfluidic devices for multiple different applications.
María Ramos Payan, J Chromatogr Sep Tech 2017, 8:5(Suppl)
DOI: 10.4172/2157-7064-C1-032