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.com
Volume 7
Clinical Microbiology: Open Access
Clinical Microbiology 2018
July 16-17, 2018
July 16-17, 2018 Melbourne, Australia
2
nd
International Conference on
Medical and Clinical Microbiology
Real-time polymerase chain reaction assay for the detection of
H. pylori
in patients with dyspepsia:
Comparison with histopathology examination
Mardiastuti
1,2
1
University of Indonesia, Indonesia
2
dr. Cipto Mangunkusumo National Central General Hospital, Indonesia
H
elicobacter
pylori infection is still a health problem in Indonesia and may lead to malignancy. Early detection may
increase the effectiveness of treatments and prevent complications. This study was aimed to determine the accuracy of
real-time Polymerase Chain Reaction (rPCR) compare to histopathology examinations. Endoscopies of antral and corporal
biopsies were performed in 34 consecutive patients with dyspepsia who did not take antibiotics and proton pump inhibitor
for two weeks (February-October 2017). The tissue biopsies were stained with HE and the rPCR was conducted using primers
previously. The thermal cycle of rPCR was 95 oC, 3’; 45 cycles of denaturation 95 oC, 15”; annealing temperature was 64 ᴼC, 1’.
The primer and probe concentration were 0.8 μM and 0.6 μM, respectively. Some of the positive specimens were sequenced to
confirm the presence of H. pylori. The minimal DNA concentration detected was 3.810-11 ng/ul. No other microbes showed
positive result. Real-time PCR revealed a higher positivity rate 32.35% (11/34) compared to histopathology examinations
20.59% (7/34). The positivity rate of rPCR from the antral was higher than from the corporal specimens. There was only one
discordance result in which histopathology showed a positive result, while the rPCR was negative. All of specimens sequenced
(7/34) were confirmed as H. pylori. Real time PCR is able to enhance the positivity rate in detecting
H. pylori
directly from
the specimen. Furthermore, rPCR was specific, sensitive, less time consuming and more cost effective than histopathology
examinations.
Biography
Mardiastuti has completed his MSc in Microbiology from Western Illinois University in 1991 and PhD in Medical Education from Gadjah Mada University in 2013.
She is the Director of Postgraduate training program of Clinical Microbiology, Faculty of Medicine, University of Indonesia. She is also the Research Coordinator at
Department of Medical Education at the same institution.
mardiastutiw@yahoo.comMardiastuti, Clin Microbiol 2018, Volume 7
DOI: 10.4172/2327-5073-C2-036