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.com
Volume 10
Journal of Cancer Science & Therapy
ISSN: 1948-5956
Euro Cancer 2018
July 23-25, 2018
July 23-25, 2018 | Rome, Italy
29
th
Euro-Global Summit on
Cancer Therapy & Radiation Oncology
Investigation of piR-36707 and piR-36741 expression levels in renal cell (transparent cell type)
carcinomas
Sercan Ergun
1, 2
, Diler Us Altay
1
, Sezgin Gunes
2
, Recep Buyukalpelli
2
and
Oguz Aydin
2
1
Ordu University, Turkey
2
Ondokuz Mayıs University, Turkey
Background:
Piwi interacting RNA (piRNA) is the main class of small non-coding RNA molecules expressed in animal cells.
Some of the piRNAs expression increases and shows oncogenic properties; while on the other hand, some of their expression
decreases and shows tumorigenic properties in specific cancer types.
Objective:
The present research is in comparison within normal and tumor renal biopsy specimens taken from patients with
clear cell renal cell carcinoma (RCC), and in kidney tissue samples of non-transfected RCC patients who were operated for
different reasons, two possible piRNAs (piR -36707 and piR-36741) expression levels, which are not related before but probably
related to RCC.
Methodology &Theoretical Orientation:
Between January 2016 and January 2017, formalin-fixed paraffin embedded (FFPE)
specimens were obtained from 19 patients who had undergone partial or radical nephrectomy for diagnostic purposes and
who had a clear cell RCC diagnosis in urology and pathology in the Ondokuz Mayıs University, Faculty of Medicine (OMÜTF).
Tumor tissue sample and a healthy tissue sample from the same patient were included in the study. FFPE was made from tissue
using a total RNA isolation kit. The piRNA-specific cDNA synthesis kit was used to convert the resulting piRNA into cDNA.
piR-36707 and piR-36741 expression levels were measured using commercially available primers and real-time PCR kit specific
to these piRNAs. Statistical significance analysis of the levels of piRNA expression was performed between the two groups.
Findings:
Expression levels of piR-36707 and piR-36741, calculated by the comparative 2-ΔΔCt curve between tumor and
normal kidney tissues, are given in comparison in Figure 1. The higher expression levels of piR-36707 and piR-36741 in
tumor tissues than normal tissues give them a potential oncogenic function. However, when this relationship was examined by
statistical methods, it was seen that p value was above 0.05 for both piRNAs and these changes were not significant.
Conclusion & Significance:
According to the literature, this is the first study that relates to clear cell RCC pathogenesis and
piR-36707 and piR-36741 genes. Prospectively, all genes in the target of piR-36707 and piR-36741 are studied as panels to
achieve universal and comprehensive data for the pathogenesis of clear cell RCC.
Figure 1:
Comparison of expression levels of piR-36741 and piR-36707 between tumor and normal kidney tissues.
Recent Publications:
1. Ciccarese C, et al. (2016) The prospect of precision therapy for renal cell carcinoma. Cancer Treatment Reviews 49:37–
44.
2. Parekh H and Rini B I (2016) Emerging therapeutic approaches in renal cell carcinoma. Expert Review of Anticancer
Therapy 15(11):1305–14.
Diler Us Altay et al., J Cancer Sci Ther 2018, Volume 10
DOI: 10.4172/1948-5956-C8-144