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Journal of Experimental Food Chemistry | Volume: 4
3
rd
International Conference on
May 16-18, 2018 | Montreal, Canada
Food Chemistry & Nutrition
Antimicrobial activity of grape seed and skin extracts coated on corona treated LDPE and PET films
Nahla Mohamed Abdel khalek Khalil
American University, Egypt
C
onsumer demand for ready-to-eat ‘fresh’ and safe food products with less synthetic preservatives together with well
documented food-borne microbial outbreaks drive both research and food industry toward new innovative methods for
microbial growth inhibition while keeping food freshness, quality, and safety. Incorporation of natural bioactive agents in the
packaging material to increase the shelf life of meat products is a promising technology. Grapes are of special interest because of
their high content of phenolic compounds that showed antimicrobial and antioxidant effects. The aim of the present work was
to investigate grape seed (GSE) and skin (GSKE) extracts’ antibacterial activity and developing bioactive LDPE/PET films that
could be used as food packaging for poultry and meat products. Commercial corona treated LDPE and PET were coated with
either grape seed or grape skin extract. Agar plate diffusion method was used for the investigation of the microbial properties of
both extracts’ coated films against
E.coli
as a Gram-negative bacterium and
Staphylococcus Aureus
as a Gram-negative one. LDPE
and PET films coated with GSE showed inhibition zones of
E.coli
growth in the range of 16-25mm, while
Staph.Aureus
growth
inhibition zones were in the range of 15-20mm. For LDPE corona films coated with grape seed extract, the minimum inhibitory
concentration (MIC) was 0.002g/1cm
2
for
E.coli
and 0.003g/1cm
2
for
Staph.Aureus
. While for corona treated PET films/GSE, the
MIC for both
E.coli
and
Staph.Aureus
was 0.002g/1cm
2
. Corona treated LDPE and PET coated with GSKE showed inhibition
zone range of 13-16.3mm with
E.coli
and 12-20mm with
Staph.aureus
. For LDPE corona films/GSKE, the minimum inhibitory
concentration (MIC) was 0.0009g/1cm2for
E.coli
and 0.003g/1cm
2
for
Staph.aureus
. While for corona treated PET films/GSKE,
the MIC was 0.002g/1cm
2
for
E.coli
and 0.003g/1cm2 for
Staph
.
Aureus
. The total phenolic content of both GSE and GSKE
was determined using the Folin- Ciocalteu methodology to be 315.32g (GAE)/kg, and 265.326g (GAE)/kg for GSE and GSKE
respectively. The coated films; LDPE/GSE or GSKE, were used to wrap fresh chicken fillets, TVC, Pseudomonads, Brochothrix
thermosphacta, Lactic acid bacteria and Enterobacteriaceae counts were determined during the storage period; ten days for test
samples and eight days for controls. Microbiological analysis for tested samples was done on day zero, two,four, six, eight, and
ten, while for control till day eight. There was a reduction in the populations of the examined bacteria in the range of 0.2-1.4 log
cfu/g in case of GSE, while with GSKE the reduction of bacterial populations range was 0.3-1.95 log cfu/g.
Biography
Nahla Mohamed Abdel khalek Khalil has completed her MS in Food Chemistry at American University in Cairo, School of Sciences and Engineering, Chemistry
Department. She is a Research Assistant at Chemistry Department, American University in Cairo. She intend to complete her research focusing on the other
important charcteristcs of both grape seed and skin extracts e.g. anticancer and antinflamatory.
nana@aucegypt.eduNahla Mohamed Abdel khalek Khalil, J Exp Food Chem 2018, Volume: 4
DOI: 10.4172/2472-0542-C1-011