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Journal of Experimental Food Chemistry | Volume: 4
3
rd
International Conference on
May 16-18, 2018 | Montreal, Canada
Food Chemistry & Nutrition
Extraction techniques of anti-nutritive and toxic factors in the leaves of the white flowering
Nerium
oleander L.
K G Kgosana
ARC- Onderstepoort Veterinary Research, South Africa
P
lant poisoning is a serious concern across the globe. However, most livestock animals in the sub-Saharan African countries
still depend on browsing on tree fodders to maintain their normal physiological processes due to high costs of animal feeds
and all resources required for feed production. Conversely, plants contain endogenous toxins commonly referred to as anti-
nutritive factors (ANFs) that often interferewith utilization of nutrients and/or feed/food intake of plants or plant products.Their
abundance frequently leads to massive clinical trauma resulting in high morbidities and mortalities. Hence, the objective of the
study was to do preliminary screening of the extraction techniques of the white flowering
Nerium Oleander
L. (Apocynaceae)
and commercial feeds. Organic (hexane, acetone and methanol) sequential and aqueous (infusion and decoction) extractions
were explored. Subsequently, a qualitative and HPLC quantitative analysis was carried out to compare contents of ANFs where
the Mann Whitney U statistical tool was used at a threshold level of 0.05. The results showed higher extraction yields in all
aqueous extractions. Therefore, an infusion may be considered as the best approach to mitigate plant poisoning due by ANFs
in plants since it proved to be an efficient, safe and reliable method. Furthermore, although the results were not so significant
(p<0.05), a high saponin content of 0.113±0.104 mg/g in diosgenin equivalent was obtained in commercial feeds. In addition,
LC-MS will be conducted to characterise the quantified ANFs from the sample.
Biography
Kedibone Gloria Kgosana has her expertise in Natural Science. During the development of her career, she explored various fields of study such as chemistry,
biochemistry, plant biotechnology and phytomedicine. After few years of experience in research and veterinary toxicology, the recent climatic change in South Africa
which had major implications on the feeding patterns of livestock in the rangelands where there is no proper management prompted her to find solutions to mitigate
the effects. Hence she developed strategies to remove or reduce the anti-nutritive and toxic factors in plants that pose a major risk in the livestock. The strategies are
responsive to all stakeholders and would improve animal health and production.
mawelakg@arc.agric.zaK G Kgosana, J Exp Food Chem 2018, Volume: 4
DOI: 10.4172/2472-0542-C1-011