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Journal of Glycobiology | ISSN: 2168-958X | Volume 7
Glycobiology & Glycoproteomics
5
th
International Conference on
&
August 27-28, 2018 | Toronto, Canada
Molecular Biology & Nucleic Acids
3
rd
International Conference on
Kinetic basis for DNA target specificity of CRISPR-Cas12a
Isabel Strohkendl
University of Texas at Austin, USA
C
lass 2 CRISPR-Cas nucleases have become the gold standard for genome editing, as genetic manipulation can be achieved
by a relatively simple and easily adaptable system1. The common class 2 CRISPR-Cas nucleases, Cas9 and Cas12a
(previously known as Cpf1), are programmable via a guide RNA that uses complementarity with DNA to identify the correct
target. Still, cleavage at off-target sites resembling the intended sequence remains a pervasive problem2. DNA targeting by
Cas12a is more specific than Cas93, but the mechanistic basis for this increased specificity is not understood. To dissect the
reaction steps of DNA targeting by Acidaminococcus sp Cas12a, we used a quantitative kinetics approach, measuring rate
constants for binding, dissociation, and DNA strand cleavage. We show that Cas12a binds DNA in two kinetically-separable
steps; protospacer-adjacent motif (PAM) recognition is followed by R-loop formation. Once it is bound, Cas12a cuts the
non-target strand repeatedly, trimming it back towards the PAM, and then it cleaves the target strand imprecisely. Because
R-loop formation is rate-limiting for cleavage, Cas12a binding inevitably leads to target DNA cleavage. Even when targeted to
DNA with single mismatches, Cas12a binding remains rate-limiting for DNA cleavage. Thus, the specificity of Cas12a for the
matched target is defined by the kinetics of binding rather than by affinity. Nevertheless, in contrast to simple expectations for
R-loop formation, we show that Cas12a retains substantial specificity against mismatches throughout the R-loop. This strong
discrimination indicates a late transition state for binding, implying that R-loop propagation is reversible up until the R-loop
has almost entirely formed. Our results provide a mechanistic foundation for the DNA cleavage patterns of Cas12a measured
in vivo 3-5 and lead to a model for the higher specificity of Cas12a than Cas9.
Biography
Isabel Strohkendl has a strong curiosity for understanding the mechanisms by which proteins with diverse structures and functions act on DNA. Under the supervi-
sion of Dr Rick Russell at the University of Texas at Austin, she has been studying protein-nucleic acid interactions and using quantitative biophysical approaches
to elucidate the mechanisms of DNA-binding enzymes. Currently, her work focuses on understanding how both nucleic acid and nucleic acid-protein interactions
dictate substrate preference during Cas12a-crRNA assembly and contribute to Cas12a specificity during DNA targeting.
istrohekendl@utexas.eduIsabel Strohkendl, J Glycobiol 2018, Volume 7
DOI: 10.4172/2168-958X-C1-012