pediatrics-pediatric-gastroenterology-2018-posters

7 / 29

Page 79
Notes:
conferenceseries .com
Volume 8 Pediatrics & Therapeutics
ISSN: 2161-0665 Pediatrics and Pediatric Gastroenterology 2018
March 21-22, 2018
16th Annual World Congress on
3rd Annual World Congress on
March 21-22, 2018 | New York, USA
PEDIATRICS
PEDIATRIC NUTRITION,
GASTROENTEROLOGY & CHILD DEVELOPMENT
&
Evidence of intra- familial of Helicobacter pylori by PCR- based RAPD fingerprinting in Vietnam
Thi-Ut Nguyen1, Thanh–Hai LE 1 and Thi-Thu –Ha Hoang2
1National Pediatric Hospital, Vietnam
2National Institute of Hygiene and Epidemiology, Vietnam
Statement of the problem: Helicobacter pylorus (H. pylori) is the major cause of gastritis and peptic ulcer disease in children.
Transmission between familiy members could be increasing risks of reinfection in children. The objective of this study is to
assess transmission by DNA fingerprinting analysis of H. pylori culture from pediatric patients and their family members.
Methodology &Theoretical Orientation: We selected 17 households with H. pylori patients. 50 H. pylori strains were isolated
from patients and family members, of whom 17 were from pediatric patients, 33 from family members including fathers,
mothers and brothers/sisters. All 50 H. pylori strains were analyzed by RAPD technique from January, 2012 through September,
2013 in National hospital of pediatrics, Hanoi, Vietnam.
Findings: Results show that 46.1% (6/13) mothers had genotypes similar to the ones of their children. In contrast, only 8.3%
(1/12) fathers had genotypes similar to the ones of their children. 11.8% (2/17) H. pylori strains of pediatric patients were
similar to the ones of their brothers/sisters. However, only one strain of fathers and mothers have similar genes but are not
identical to their offspring.
Conclusion & Significance: The results show that there is a link between the mother-to-child isolate, which may be evidence
of mother-to-child transmission, especially in young children. The results of our study provide further proof of the molecular
level of H. pylori transmission between family members in Vietnam. Further studies on H. pylori transmission among family
members with larger sample sizes are needed.
Biography
Thi-Ut Nguyen is a Pediatrician who has been working as Gastroenterologist at Gastroenterology Department of the National Pediatric Hospital. She has conducted
several studies on Helicobacter pylori antibiotics resistance and virulence of gastritis and peptic ulcer among children.
Thi-Ut Nguyen et al., Pediatr Ther 2018, Volume: 8
DOI: 10.4172/2161-0665-C1-049