world-obesity-2018-posters-abstracts

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Page 33
Volume 8
Oct 25-26, 2018 Budapest, Hungary
21 st World Obesity Conference
World Obesity-2018 Journal of Obesity & Weight Loss Therapy | ISSN : 2165-7904
Effect of melanocortin obesity on insulin signaling in female and male
mice
In the human population, melanocortin obesity is more common than other forms of
genetic types of obesity. In mice mutation “Yellow” in the Agouti locus (Ay) decreases
melanocortin receptor activity, induces hyperphagia, adult onset obesity and insulin
resistance. It remains unknown if there are sex differences in molecular mechanisms of
insulin resistance at melanocortin obesity. We investigated effects of melanocortin obesity
on adipose tissue, glucose tolerance, insulin sensitivity, insulin and glucose blood levels,
and mRNA level of insulin signaling genes (INSR, IRS1/2, PIK3CD) in liver, muscle
and adipose tissue (WAT) in females and males at 30 weeks of age. We have shown that
both Ay-females and Ay-males have impaired whole body insulin sensitivity: females had
glucose intolerance and fed hyperinsulinemia, and males had impaired insulin sensitivity
and fasted hyperinsulinemia. However, the molecular mechanisms of insulin resistance
were different in Ay-females and Ay-males. We found sex specific effects of melanocortin
obesity on insulin signaling in liver and adipose tissue, whereas obesity did not affect
insulin signaling in muscles. In females, obesity was associated with decreased hepatic
INSR and IRS2 mRNA levels, and increasedWAT INSR and PIK3CD mRNA levels, and in
males, obesity was associated with increased hepatic PIK3CD mRNA level and decreased
WAT INSR mRNA level. The data suggest that sexual differences in mechanisms of insulin
resistance should be considered for correction of metabolic syndrome at melanocortin
obesity.
This study was supported by the Russian Science Foundation, Grant No 17-15-01036.
Biography
Iakovleva T V has graduated from Novosibirsk University. At the University, she studied the properties and
function of estradiol secreted by the adrenal gland. She then studied effects of color mutations (Agouti and non-
Agouti) on the function of pituitary-adrenal system in females and males of Arvicola terestris; effects of colour
mutation Agouti yellow on the function of pituitary-adrenal system in mice C57Bl and; effects of melanocortin
system and estradiol on obesity development and insulin sensitivity in females of C57Bl mice. She studied
mechanisms underlying the development of insulin resistance and expression of insulin signal transduction
genes in females and males of C57Bl mice during the development of melanocortin and diet-induced obesity
and presented work is the part of her study on sex characteristics of the FGF21 regulatory effects.
Iakovleva T V
The Institute of Cytology and
Genetics, Russian Federation
Co-Authors
Makarova E N and
Bazhan N M
The Institute of Cytology and
Genetics, Russian Federation
Iakovleva T V, J Obes Weight Loss Ther 2018, Volume:8
DOI: 10.4172/2165-7904-C10-085