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Page 35
Volume 8
Oct 25-26, 2018 Budapest, Hungary
21
st
World Obesity Conference
World Obesity-2018
Journal of Obesity & Weight Loss Therapy | ISSN : 2165-7904
Sex-dependent differences of mineralocorticoid system activity in
melanocortin obesity in Ay mice
A
gouti yellow mice (Ay) with melanocortin obesity is a convenient model for studying
of the molecular mechanisms of non-dietary type of obesity. It has been shown
recently that mineralocorticoid system played an important and sex dependent role in
obesity development. Complications associated with it often led to the chronic heart and
renal failure. However, sex dependent characteristics of the mineralocorticoid system
activity at this type of obesity have not been adequately studied. The aim of this work was
to investigate the expression of mineralocorticoid receptor (MR) and aldosterone level in
female and male Ay mice. Using the real-time PCR method, the mRNA level of the MR in
the hypothalamus, heart left ventricle and adipocytes in adult female and male Ay mice at
the age of 30-32 weeks was investigated. The level of aldosterone in the blood was studied
using the enzyme immunoassay method (Mouse Aldosterone (ALD) ELISA kit). Twice, as
high level of MR mRNA has been detected in female adipocytes and in heart left ventricle
in female Ay mice compared to male (p≤0.05). No significant differences in MR mRNA
level in the pituitary glands have been identified. Sex dependent difference in the blood
aldosterone levels (224.6±25.1 and 102.8±16.5 pg/ml in the female and male Ay, p≥0.05)
has been shown. These data indicate that melanocortin obesity in female Ay is associated
with aldosterone and mineralocorticoid system activation.
The study was supported by the RFBR (17-04-00912) and Budget Project № 0324-2018-
0016
Biography
N S Logvinenko is a Senior Scientist at the Institute of Cytology and Genetics, Russian Academy of Sciences,
Novosibirsk. Her main fields of scientific interests are: (1) Molecular mechanisms of aldosterone regulation of
kidney function during postnatal ontogenesis; (2) The effects of kinase phosphorylation of the alpha-subunit
of kidney Na+-K+-ATPase on the biological activity of the enzyme; (3) Nongenomic aldosterone effects on the
kidney ENaC activity and (4) Investigation of the mineralocorticoid receptors and aldosterone nongenomic
effects in Ay mice with melanocortin obesity.
ninlo@bionet.nsc.ruN S Logvinenko
Institute of Cytology and
Genetics, Russian Federation
Co-Authors
L E Katkova
and
G S Baturina
Institute of Cytology and
Genetics, Russian Federation
N S Logvinenko et al., J Obes Weight Loss Ther 2018, Volume:8
DOI: 10.4172/2165-7904-C10-085