Adsorption Chromatography

Adsorption chromatography is definitely one of the earliest types of analytical method of separation technology. Adsorption means a physical connection between the compound and the particles of stationary phase. It utilizes a mobile liquid or gaseous phase that is adsorbed onto the top most layer of an immobilized solid phase. Column chromatography has been employed to separate vitamins, steroids, hormones, and alkaloids and to determine the amounts of these substances in samples of body fluids .The different separation techniques involved are column chromatography, thin layer chromatography and liquid chromatography

The applications of chromatography are in qualitative and quantitative analysis of low polarity compounds and also measuring toxic substance in air, soil or water

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Adsorption chromatography applies to only solid-liquid or solid-gas chromatography. Because the adsorption phenomenon is inherent property of solids and hence it is used with only solid stationary phase chromatographs.Liquid chromatography is one of the important chromatographic technique used widely in the pharmaceutical industry.

  • Column chromatography
  • Thin layer chromatography
  • Liquid chromatography
  • Principle involved in adsorption chromatography

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