Advancement in Chromatography

Chromatography, different method for identifying and separating the components, or solutes, of a mixture on the basis of the relative amounts of each solute distributed between a moving fluid streams, called the mobile phase, and a contiguous stationary phase. Liquid-chromatography is one of the mostly employed and useful techniques in pharmaceutical industry for sample preparation. Another one is electrophoretic separation technique designed for rapid and selective sample analysis. Immunoaffinity chromatography (IAC) is a different method of Liquid Chromatography in which the stationary phase consists of an antibody or it might include antibody-related reagent. This technique involves a unique sub type of affinity chromatography, in which a biologically related binding agent is taken for the selective separation or analysis of a target compound. Capillary electrophoresis is other method which identifies and distributes ions depending on their electrophoretic mobility with the use of an applied voltage. Compact mass spectrometer is widely used in the food safety, water purity and clinical diagnosis.

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Recent Advances in Chromatography involves capillary electrophoresis, multidimensional chromatography, High-performance and Immuno-affinity chromatography, fluorescence chromatography, optical force chromatography, flash chromatography and electrophoretic separations.Unlike flow cytometry, optical chromatography based on optical force separation of particle types does not require a fluorescent or antibody tag, but relies on the balance of optical and fluidic forces on a particle in a microchannel. The concept of optical force, in which a laser or light beam exerts a fraction of its momentum on a particle through scattering, refraction, or other particle interactions, is well known in the biomedical community through techniques such as optical tweezing/trapping and optical lift.

  • Multidimensional chromatography
  • High-performance and Immuno-affinity chromatography
  • Fluorescence derivatization in HPLC
  • Optical force chromatography
  • Flash chromatography
  • Solid-phase micro extraction gas chromatography–Mass spectrometry

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