Advances in Chromatography & HPLC Techniques

HPLC is a popular method of analysis for natural products because of its high accuracy, precision and is not differed by the stability or the volatility of the compounds. HPLC combined with diode array detector (HPLC-DAD), mass spectrometer (HPLC-MS) have been successfully utilized for the qualitative and quantitative determination of various types of phytoconstituents like alkaloids, glycosides, tannins, triterpenes, flavonoids etc. HPLC methods are used readily for the determination of drug in biological fluids and pharmaceutical dosage forms. HPLC determination with spectroscopic detection is useful for routine quality control of drugs in pharmaceutical dosage forms and stability studies.

A chromatographic detector is capable of establishing both the identity and concentration of eluting components in the mobile phase stream. A broad range of detectors is available to meet different sample requirements. Detectors respond to a particular compound only and the response is independent of mobile phase composition and the response of bulk property detectors is dependent on collective changes in the composition of sample and mobile phase. Specific detectors are UV-VIS, Photodiode array, fluorescence, and mass spectroscopic detectors. Bulk Property detectors include refractive index, electrochemical and light scattering detectors.

  • Advances in Chromatography-HPLC Instrumentations
  • HPLC Fingerprinting in Bioinformatics and Computational Biology
  • Chromatography-HPLC Separation Techniques

HPLC is a popular method of analysis for natural products because of its high accuracy, precision and is not differed by the stability or the volatility of the compounds. HPLC combined with diode array detector (HPLC-DAD), mass spectrometer (HPLC-MS) have been successfully utilized for the qualitative and quantitative determination of various types of phytoconstituents like alkaloids, glycosides, tannins, triterpenes, flavonoids etc. HPLC methods are used readily for the determination of drug in biological fluids and pharmaceutical dosage forms. HPLC determination with spectroscopic detection is useful for routine quality control of drugs in pharmaceutical dosage forms and stability studies.

A chromatographic detector is capable of establishing both the identity and concentration of eluting components in the mobile phase stream. A broad range of detectors is available to meet different sample requirements. Detectors respond to a particular compound only and the response is independent of mobile phase composition and the response of bulk property detectors is dependent on collective changes in the composition of sample and mobile phase. Specific detectors are UV-VIS, Photodiode array, fluorescence, and mass spectroscopic detectors. Bulk Property detectors include refractive index, electrochemical and light scattering detectors.

  • Advances in Chromatography-HPLC Instrumentations
  • HPLC Fingerprinting in Bioinformatics and Computational Biology
  • Chromatography-HPLC Separation Techniques

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