Advances in HPLC Instrumentation

HPLC is a popular method of analysis for herbal products because of its accuracy, precision and not limited by the volatility or stability of the sample compounds. HPLC combined with diode array detector (HPLC-DAD), mass spectrometer (HPLC-MS) have been successfully employed in the qualitative and quantitative determination of various types of phytoconstituents like flavonoids, alkaloids, tannins, triterpenes, glycosides, sterols etc. HPLC methods are used in the determination of drug in biological fluids and pharmaceutical dosage forms. HPLC determination with spectroscopic detection is useful for routine quality control of drugs in pharmaceutical dosage forms and stability studies. Several synthetic molecules also are successfully evaluated both qualitatively and quantitatively. Pharmaceutically important antiviral drugs (lamivudine, stavudine, nevirapine, zidovudine), NSAIDs (acetaminophen, meloxicam, paracetamol, phenylbutazone,), antibiotics (amoxicillin, cefepime, rifampicin), CNS stimulants (amphetamine), betablockers (atenolol), preservatives (benzalkoniumchloride), Diuretics (hydrochlorthiazide), antidiabetics (metformin), anticancer (methotrexate), corticosteroids (prednisone, betamethasone, dexamethasone), anti-allergic (cetrizine), antidepressant (Nitroxazepine, milnacipran), veterinary anaesthetic (ketamine) , anti-inflammatory (sodium osagrel), secretolytic (ambroxol) and several other category drugs were successfully evaluated via HPLC.

HPLC columns are usually packed with porous, or pellicular particles. Pellicular particles are made from polymers, or glass. Pellicular particles are surrounded by a thin uniform layer of silica, synthetic resins, alumina, or an of ion-exchange resin. Partition HPLC uses liquid bonded phase columns. The liquid stationary phase is chemically bonded to the packing material. The packing material is usually hydrolysed silica which reacts with the bond-phase coatings like siloxanes. A chromatographic detector is capable of establishing both the identity and concentration of eluting components in the mobile phase stream. A broad range of detectors are available to meet different sample requirements. Detectors respond to a particular compound only and the response is independent of mobile phase composition and the response of bulk property detectors is dependent on collective changes in composition of sample and mobile phase. Specific detectors are UV-VIS, Photo diode array, fluorescence, and mass spectroscopic detectors. Bulk Property detectors include refractive index, electrochemical and light scattering detectors.

HPLC instrumentation primely includes Columns and Detectors. Columns are either coventional particle packed or the latest bonded phase and monoliths. Detectors are developed such that almost all the chemical moieties are identified with lease chances of errors.

  • Advances in qualitative and quantitative analysis
  • Advances in column packing design
  • Advances in HPLC detectors

Related Conference of Advances in HPLC Instrumentation

August 10-12, 2017

8th Asian Biologics and Biosimilars Congress

Beijing, China
September 20-21, 2017

10th Pharmacovigilance Congress

Charlotte, North Carolina, USA
September 25-26, 2017(10 Plenary Forums- 1 Event)

7th International Conference and Exhibition on Pharmaceutical Regulatory Affairs and IPR

Chicago, Illinois, USA
September 25-26, 2017
(10 Plenary Forums - 1Event)

6th International Conference and Exhibition on GMP, GCP & Quality Control

Chicago, Illinois, USA
October 02-04, 2017

World Congress on Antibiotics: R&D, Market

Atlanta, USA
October 16-18, 2017

12th World Pharma Congress

Budapest, Hungary
October 16-18, 2017

11th World Drug Delivery Summit

(10 Plenary Forums - 1Event)
Baltimore, Maryland, USA
October 16-17, 2017

10th International Conference and Exhibition on Biologics and Biosimilars

San Francisco, California, USA
November 09-11, 2017

4th European Biopharma Congress

Vienna, Austria

Advances in HPLC Instrumentation Conference Speakers

Recommended Sessions

Related Journals

Are you interested in