Affinity Chromatography

Affinity chromatography is one of the important separation techniques based on a specific binding interaction between an immobilized ligand and its binding partner. Affinity chromatography is a type of liquid chromatography that makes use of interactions for the identification and specific analysis of sample components. Examples include antibody and antigen, enzyme and substrate, and enzyme and inhibitor interactions. The degree of purification can be quite high in a purification strategy. This present track provides an knowledge of affinity chromatography of proteins. Immunoaffinity chromatography is one of the important types of affinity chromatography. Immunoaffinity chromatography technique is more commonly performed for the identification, quantification, or purification of antigens.

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Since the success of affinity chromatography resides in its ability to bind an active site to its corresponding ligand, if the protein binding region cannot join with the immobilized ligand the technique is effectively useless. Spacer arms, though not always necessary, can improve binding probability. Spacer arms distance the ligand from the matrix reducing steric hinderance which can occur when the ligand is bound directly to the bead. Spacer arms should neither chemically or structually affect the sample or the ligand.

  • Immunoaffinity chromatography
  • Immobilized metal ion affinity chromatography
  • Mechanism of Affinity chromatography
  • Protein affinity chromatography
  • Affinity column chromatography
  • Metal affinity chromatography

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