Amino Acids Analysis and Trends
HPLC is the most popular method for analyzing amino acid components, which have currently gained attention due to the boom in health foods.
Using UV detection for amino acids in most cases requires using the absorption of the carboxyl group (-COOH) in the 200 to 210 nm range. Some amino acids with benzene rings can also be detected in the 250 to 280 nm range, but in general, they are difficult to analyze as-is with sufficient sensitivity and selectivity.
Consequently, derivatization methods have long since been used. Since many amino acids contain amino groups (-NH2and -NHR) in their structures, a derivatizing reagent that selectively reacts with the amino group is used.
Pre-column Derivatization Method: In pre-column derivatization, the amino acids are derivertized before injection, and then the reaction products are separated and detected.
Post-column Derivatization Method: The post-column derivatization method involves separating the amino acids in the column, then delivering and mixing the derivatizig reagent to let it react with the amino acids, before finally sending the products to the detector.
- High performance liquid chromatography
- Pre-column derivatization method
- Post-column derivatization method
Related Conference of Amino Acids Analysis and Trends
Amino Acids Analysis and Trends Conference Speakers
- Amino Acids Analysis and Trends
- Animal Biochemistry
- Bio-organic Chemistry
- Cell Biology
- Chemical Ecology
- Chemotaxonomy & Virology
- Clinical Glycomics
- Enzymology & Immunology
- Forensic Science/Biochemistry
- Glycomic diseases
- Glycomic immunotherapies
- Glycoproteomics and Protein Expression
- Lipid Health and Nutrition
- Mathematical Biology
- Metabolic Biochemistry
- Molecular Biology
- Molecular genetics and Genetic engineering
- Obesity and Diabetes
- Pharmaceutical Biochemistry
- RNA Biology
- Stem Cell
- Structural Biochemistry
- Xenobiotics and Endocrinology