Antibody Humanization Technologies

The first monoclonal antibodies were typically made entirely from mouse cells. One problem with this is that the human immune system will see these antibodies as foreign (because they’re from a different species) and will mount a response against them. In the short term, this can sometimes cause an immune response. In the long term, it means that the antibodies may only work the first time they are given; after that, the body’s immune system is primed to destroy them before they can provide treatment. This study presents a technology that generates stable, soluble, ultra-humanized antibodies via single-step CDR redundancy minimization. Lead clones demonstrated high stability, with affinity and specificity equivalent to, or better than, the parental immunoglobulin. This significantly lowered non-human sequence content, minimized t- and b-cell epitope risk in the final molecules and provided a heat map for the essential non-human CDR residue content of antibodies from disparate sources. Antibody humanization uses multiple sequence segments derived from variable (V) regions of unrelated human antibodies, unlike other technologies that typically use a single human V region framework as acceptors for complementarity determining regions (CDRs) from starting antibodies (typically rodent). Through careful selection of human sequence segments and the application of in silico tools, CD4+ T cell epitopes are avoided so the risk of immunogenicity is reduced compared to standard humanized antibodies whilst antibody affinity and specificity is maintained. Immunogenicity assessment technology is used to confirm T cell epitopes have been removed.

We can provide small quantities of antibody for research purposes using either transient expression systems or through generating research-grade stable cell lines. For larger quantities for development a stable high-expressing manufacturing cell line can be established using our cell line development service and then transferred to a cGMP compliant manufacturer.

  • Rapid Humanization and Affinity Improvement of a Murine Antibody
  • IMGT Databases and Tools for Antibody Engineering and Humanization
  • Immunogenicity Assessment Strategies to Support the Development of Biological Therapeutics
  • Therapeutic Antibody Discovery and Development Using Humanized RabMAbs
  • Humanized Antibodies and Their Therapeutic Value
  • Non-human Primate Immune Libraries Combined with Germline Humanization
  • A Novel Fully Human Monoclonal Antibody Platform Using Transgenic Rats

Related Conference of Antibody Humanization Technologies

May 16-17, 2018

World Congress on Allergy and Immunotherapy

Osaka, Japan
September 13-14, 2018

World Conference on Vaccine and Immunology

Bucharest, Romania
September 13-14, 2018

11th Annual Congress on Immunology & Immunotechnology

Zurich, Switzerland
September 17-18, 2018

3rd International Conference on Tumor & Cancer Immunology and Immunotherapy

San Diego, California, USA
September 17-18, 2018

3rd International Conference on Tumor & Cancer Immunology and Immunotherapy

San Diego, California, USA
September 20-21, 2018

Global Meet on Immunology and Molecular Biology

Oslo, Norway
October 19-20, 2018

10th World Congress and Expo on Immunology

New York, USA
October 29-30, 2018

6th International Conference on HIV/AIDS, STDs and STIs

San Francisco, USA
November 26-27, 2018

3rd International Conference on Autoimmunity

Dublin, Ireland
December 06-07, 2018

Annual Congress and Expo on Antibiotics

Amsterdam, Netherlands
March 04-05, 2019

10th Molecular Immunology & Immunogenetics Congress

Barcelona, Spain

Antibody Humanization Technologies Conference Speakers

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