Biochemical Applications of Chromatography

Chromatography-HPLC is a very common method for metabolomics analysis. With the invention of electrospray ionization, HPLC is coupled to mass spectroscopy. HPLC has lower chromatographic resolution, requires no derivation for polar molecules and separates molecules in the liquid phase. HPLC has the advantage of much wider range of analytes measurements with a higher sensitivity than gas chromatographic methods. Relevant to proteomics, due to the complex structure and nature of proteins, instrumentation and methods development for sample clean-up, pre-concentration, fractionation, chromatographic separation and detection becomes an immediate requirement for the identification of peptides and proteins. Latest techniques and equipment for separation and detection include nano-HPLC and multidimensional HPLC for protein and peptide separation. HPLC is considered as most reliable and most sensitive technique in genomics used to determine DNA methylation. The nucleosides and nucleotides of DNA are separated and quantified by HPLC-UV method. HPLC finds applications in glycomics and lipidomics where glycan part is cleaved either enzymatically or chemically from the target and subjected to analysis. HPLC has a wide application in lipidomics to separate lipids prior to mass spectrometry. Separation can be achieved by either reverse-phase (RP) HPLC or normal-phase (NP) HPLC.

  • Proteomics
  • Lipidomics
  • Clinical Diagnosis
  • Nano Technology
  • Biopharmaceutical data screening

Related Conference of Biochemical Applications of Chromatography

October 30-31, 2019

26th World Congress on Chemistry

London, UK
November 18-19, 2019

World Polymer Chemistry & Biopolymers Congress

Rome, Italy
April 13-14, 2020

6th International Conference on Polymer Chemistry

Prague | Czech Republic
March 18-19, 2020

16th European Organic Chemistry Congress

London, UK
March 23-24, 2020

8th Global Conference on Mass Spectrometry

Osaka, Japan
March 27-28, 2020

Global Conference on Chemistry and Catalysis

Amsterdam, Netherlands

10th World Congress on Chromatography

April 16-17, 2020 Amsterdam | Netherlands
April 17-18, 2020

Green & Industrial Chemistry Congress

London, United Kingdom

9th World Congress on Mass Spectrometry

June 10-11, 2020 | Berlin, Germany
July 09-10, 2020

11th World Congress on Green Chemistry and Technology

Geneva, Switzerland
July 15-16, 2020

CSBC Conference 2020

July 15-16, 2020

10th European Chemistry Congress

Vienna, Austria
July 20-21, 2020

11thInternational Conference on Chemistry

Florence Italy
October 12-13, 2020

International Conference on Petrochemistry and Natural Gas

| Prague, Czech Republic
November 22-23, 2020

Analytical Chemistry & Drug Development

Dublin,Ireland
November 23-24, 2020

19thInternational Conference on Industrial Chemistry

Zurich, Switzerland

Biochemical Applications of Chromatography Conference Speakers

Recommended Sessions

Related Journals

Are you interested in