Disease Interrogation and Applications of RNA-Seq

Disease interrogation & clinical applications of RNA-Seq involves different methods that are required for gene expression analysis. Like, we can find out the Alternative Protein-Coding Transcripts in Prostate Cancer to interrogate its nature and occurrence. Targeted RNA sequencing is a highly accurate and specific method for measuring expression of transcripts of interest, offering both quantitative and qualitative information. This allows for differential expression analysis, as well as allele-specific expression measurement and verification of the presence of fusion genes. The ability to obtain a complete picture of Intergenic Disease-Associated Regions provides clear understanding of how the underlying genome is converted into the functional proteins, allowing for clinical utility in patient classification, diagnosis, and individualized treatment. To understand which coding and non-coding RNAs are transcribed in the diseased cell, Deciphering the Diseased Transcriptome by RNA-Seq and qPCR can be done. The Northwestern University Feinberg School of Medicine has a special unit called Center for Genetic Medicine where bioinformatics analysis of a RNA-seq is performed. Other universities include The Rockefeller University and University of Bristol, UK
  • Deciphering the Diseased Transcriptome by RNA-Seq and qPCR
  • Targeted RNA Sequencing
  • RNA-Seq for Plants
  • Intergenic Disease-Associated Regions
  • RNA-Seq to Identify and Characterize Different Cancer Specific Transcripts
  • Alternative Protein Coding Transcripts in Prostate Cancer

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