Electrophoresis

The movement of particles under spatially uniform electric field in a fluid is
called electrophoresis.In 1807, Ferdinand Frederic Reuss observed clay
particles dispersed in water to migrate on applying constant electric field for the
first time. It is caused by a charged interface present between the particle surface
and the surrounding fluid. The rate of migration of particle depends on the
strength of the field, on the net charge size and shape of the molecules and also
on the ionic strength, viscosity and temperature of medium in which the
molecules are moving. As an analytical tool, electrophoresis is simple, rapid and
highly sensitive. It is used analytically to study the properties of a single charged
species and as a separation technique. It provides the basis for a number of
analytical techniques used for separating molecules by size, charge, or binding
affinity. 
  • Gel electrophoresis
  • Capillary electrophoresis
  • Affinity electrophoresis

 

The movement of particles under spatially uniform electric field in a fluid is
called electrophoresis.In 1807, Ferdinand Frederic Reuss observed clay
particles dispersed in water to migrate on applying constant electric field for the
first time. It is caused by a charged interface present between the particle surface
and the surrounding fluid. The rate of migration of particle depends on the
strength of the field, on the net charge size and shape of the molecules and also
on the ionic strength, viscosity and temperature of medium in which the
molecules are moving. As an analytical tool, electrophoresis is simple, rapid and
highly sensitive. It is used analytically to study the properties of a single charged
species and as a separation technique. It provides the basis for a number of
analytical techniques used for separating molecules by size, charge, or binding
affinity. 
  • Gel electrophoresis
  • Capillary electrophoresis
  • Affinity electrophoresis

 

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