Enzyme engineering

Enzyme engineering involves immobilized or stabilized enzymes, new classes of enzymes (ribozymes) or new enzymatic routes that produce important organic compounds. Enzymes are biological catalysts (proteins) poised to replace inorganic catalysts, which are used in chemical industry. Proteins are abundant biological entities made up of twenty amino acids strung together by a special type of thread- a chemical bond called the peptide bond. One protein differs from another in the total number of amino acids and their sequence in the chain. Enzymes are Nature’s catalysts, tremendously accelerating the rates of a wide range of biochemical reactions, often with exquisite specificity. Harnessing enzymes for other purposes usually requires engineering them to improve their activity or stability. One approach to engineering enzymes is to make specific modifications, but this demands a detailed and frequently unattainable understanding of the relationship between sequence and function.

  • Rational protein design
  • Enzyme pro drug therapy
  • Mutagenesis
  • DNA Shuffling

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