Fungal Identification and quantification

Fungal infections pose unique challenges to molecular diagnostics; fungal molecular diagnostics consequently lags behind bacterial and viral counterparts. Nevertheless, fungal infections are often life-threatening and early detection and identification of species is crucial to successful intervention. A high throughput PCR-based method is needed that is independent of culture, is sensitive to the level of one fungal cell per milliliter of blood or other tissue types, and is capable of detecting species and resistance mutations.

Because identification using morphological characters alone was frequently criticized among mycologists, we do not recommend using this approach alone for isolates with unknown identity. DSMZ offers standard tests to determine morphological (macro and micro) and physiological (growth rates, media) characters of fungi. These include development of a fungus on standard media, examination of sexual reproduction, and determination of growth characteristics. Depending on a taxonomical group, a few additional tests could be added to comply with the current practice.

  • Classic methods of fungal identi¬cation and quanti¬cation, and of their toxigenic ability
  • Measurement of fungal germination, growth and inactivation
  • Modern methods of fungal identi¬cation and quanti¬cation, and of their toxigenic ability
  • Molecular methods
  • Modern methods of fungal identi¬cation and quanti¬cation
  • Mass spectrometry (MALDI-TOF ICMS)

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