HPLC Separation Techniques

High Performance Liquid Chromatography is a non-destructive procedure for resolving a complex mixture into its individual fractions or compounds. It is based on differential migration of solutes with the solvents. The solutes in a mobile phase passes over a stationary phase. Those high affinity solutes of the mobile phase will spend more time in this phase than the solutes that prefer the stationary phase. As the solute rises up through the stationary phase they gets separated. This process is called chromatographic development. The fraction with greater affinity to stationary layer travels slower and shorter distance while that with less affinity travels faster and longer.

  • Normal Phase Chromatography
  • Reverse Phase Chromatography
  • Flash Column Chromatography
  • Ion Exchange Chromatography
  • Affinity Chromatography
  • Chiral Chromatography
  • Size Exclusion Chromatography

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