Hyphenated HPLC Methods

The hyphenated technique is developed from the coupling of a separation technique and an on-line spectroscopic detection technology. Several remarkable improvements in hyphenated analytical methods over the last two decades have significantly broadened their applications in the analysis of biomaterials, especially natural products, pre-isolation analyses of crude extracts or fraction from various natural sources, isolation and detection of natural products, chemical fingerprinting, and metabolomics. Techniques like HPLC coupled to NMR (Nuclear Magnetic Resonance) or electrospray ionization tandem mass spectrometry (ESI-MS-MS) have been proven to be extremely powerful tools in natural product analysis, as they aid in the fast screening of crude natural product extracts or fractions for detailed information about metabolic profiles, with minimum quantity of material. The application of various hyphenated techniques even allows the discovery of new molecules, including complete and conclusive structure elucidation, and relative configurations as compared to time-consuming and costly isolation and purification processes. Hyphenated HPLC techniques include HPLC-MS, HPLC-ESI-MS, HPLC-IC-MS, HPLC-NMR-MS, HPLC-DAD, HPLC-CE-MS, HPLC-UV, Coupling LC and MALDI-TOF. MALDI (Matrix Assisted Laser Desorption Ionization) is a very sensitive technique for determining the mass of proteins, polymers and peptides. MALDI basically used in protein identification. MALDI sample preparation is fast and easy and therefore a primary choice in proteomics.

  • Electrospray Ionization Tandem Mass Spectrometry(ESI-MS-MS)
  • Matrix Assisted Laser Desorption Ionization (MALDI)
  • Gas Chromatography-Mass Spectrometry(GC-MS)
  • Liquid Chromatography-Mass Spectrometry(LC-MS)
  • Pyrolysis-Gas Chromatography-Mass Spectrometry

Related Conference of Hyphenated HPLC Methods

September 04-06, 2017

5th Global Chemistry Congress

London, UK
September 11-12, 2017

3rd World Chemistry Conference

Dallas, Texas, USA
September 25-27, 2017

5th International Conference on Current Trends in Mass Spectrometry and Chromatography


(10 Plenary Forums - 1Event)
Atlanta, Georgia, USA
September 28-29, 2017
(10 Plenary Forums-1 Event)

2nd International Conference on Clinical Chemistry and Laboratory Medicine

Atlanta, Georgia, USA
October 02-04, 2017

2nd International Conference on Pharmaceutical Chemistry

Barcelona, Spain
(10 Plenary Forums-1Event)
November 02-03, 2017

18th World Conference and Exhibition on Analytical & Bioanalytical Chromatographic Techniques

Atlanta, Georgia, USA
November 15-16, 2017

2nd International Conference on Nuclear Chemistry


(10 Plenary Forums - 1Event)
Las Vegas, Nevada, USA
November 16-17, 2017

World Congress on Geochemistry

February 19-20, 2018

EuroSciCon Conference on Chemistry

Paris, France
March 01 - 03, 2018

4th European Organic Chemistry Congress

London, UK

8th European Chemistry Congress

June 11-13, 2018 Paris, France

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