Hyphenated HPLC Methods

The hyphenated technique is developed from the coupling of a separation technique and an on-line spectroscopic detection technology. Several remarkable improvements in hyphenated analytical methods over the last two decades have significantly broadened their applications in the analysis of biomaterials, especially natural products, pre-isolation analyses of crude extracts or fraction from various natural sources, isolation and detection of natural products, chemical fingerprinting, and metabolomics. Techniques like HPLC coupled to NMR (Nuclear Magnetic Resonance) or electrospray ionization tandem mass spectrometry (ESI-MS-MS) have been proven to be extremely powerful tools in natural product analysis, as they aid in the fast screening of crude natural product extracts or fractions for detailed information about metabolic profiles, with minimum quantity of material. The application of various hyphenated techniques even allows the discovery of new molecules, including complete and conclusive structure elucidation, and relative configurations as compared to time-consuming and costly isolation and purification processes. Hyphenated HPLC techniques include HPLC-MS, HPLC-ESI-MS, HPLC-IC-MS, HPLC-NMR-MS, HPLC-DAD, HPLC-CE-MS, HPLC-UV, Coupling LC and MALDI-TOF. MALDI (Matrix Assisted Laser Desorption Ionization) is a very sensitive technique for determining the mass of proteins, polymers and peptides. MALDI basically used in protein identification. MALDI sample preparation is fast and easy and therefore a primary choice in proteomics.

  • Electrospray Ionization Tandem Mass Spectrometry(ESI-MS-MS)
  • Matrix Assisted Laser Desorption Ionization (MALDI)
  • Pyrolysis-Gas Chromatography-Mass Spectrometry
  • Liquid Chromatography-Mass Spectrometry(LC-MS)
  • Gas Chromatography-Mass Spectrometry(GC-MS)

Related Conference of Hyphenated HPLC Methods

October 14-15, 2019

12th Global Experts Meeting on Chemistry and Drug Design

Seoul, South Korea
October 30-31, 2019

26th World Congress on Chemistry

London, UK
November 18-19, 2019

10th World Meet on Analytical Chemistry & Instrumentation

Bangkok, Thailand
November 18-19, 2019

World Polymer Chemistry & Biopolymers Congress

Rome, Italy
April 13-14, 2020

6th International Conference on Polymer Chemistry

Prague | Czech Republic
March 18-19, 2020

16th European Organic Chemistry Congress

London, UK
March 23-24, 2020

8th Global Conference on Mass Spectrometry

Osaka, Japan
March 27-28, 2020

Global Conference on Chemistry and Catalysis

Amsterdam, Netherlands

10th World Congress on Chromatography

April 16-17, 2020 Amsterdam | Netherlands
April 17-18, 2020

Green & Industrial Chemistry Congress

London, United Kingdom

9th World Congress on Mass Spectrometry

June 10-11, 2020 | Berlin, Germany
July 09-10, 2020

11th World Congress on Green Chemistry and Technology

Geneva, Switzerland
July 15-16, 2020

CSBC Conference 2020

July 15-16, 2020

10th European Chemistry Congress

Vienna, Austria
July 20-21, 2020

11thInternational Conference on Chemistry

Florence Italy
October 12-13, 2020

1st International Conference on Petrochemistry and Natural Gas

| Prague, Czech Republic
November 22-23, 2020

Analytical Chemistry & Drug Development

Dublin,Ireland
December 07-08, 2020

10th World Congress on Mass Spectrometry & Analytical Techniques

Prague | Czech Republic

Hyphenated HPLC Methods Conference Speakers

Recommended Sessions

Related Journals

Are you interested in