Hyphenated SeperationTechniques

 A hyphenated separation technique refers to the coupling of two or more techniques to separate chemicals from solutions and to detect them. There is a remarkable improvement in the hyphenated analytical methods over the last two decades have significantly broadened their applications in the analysis of biomaterials, especially natural products. The advancement  in the applications of various hyphenated techniques include pre-isolation analyses of crude extracts or from various natural sources, isolation and on-line detection of natural products, chemotaxonomic studies, chemical fingerprinting, quality control of herbal products, de-replication of natural products, and metabolomics studies. These techniques are also used as the separation tool. Hyphenated techniques are widely used in chemistry and biochemistry.

  • Gas Chromatography-Mass Spectrometry (GC-MS)
  • Liquid Chromatography–Mass Spectrometry (LC-MS)
  • Liquid Chromatography-Infrared Spectroscopy (LC-IR)
  • HPLC/Electrospray Ionization-Mass Spectrometry (HPLC/ESI-MS)
  • Chromatography-Diode-Array Detection (LC-DAD)
  • Capillary Electrophoresis-Mass Spectrometry (CE-MS)
  • Capillary Electrophoresis-Ultraviolet-Visible Spectroscopy (CE-UV)
  • Ion-Mobility Spectrometry–Mass Spectrometry
  • Prolate Trochoidal Mass Spectrometer

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