Innovative Fractionation & Enrichment Techniques

Phosphoproteins are often present in quite low abundance and tend to co-exist with their unphosphorylated isoform within the cell. To make their identification more practicable, the use of enrichment protocols is often required. The enrichment strategies can be performed either at the level of phosphoproteins or at the level of phosphopeptides. Both approaches have their advantages and disadvantages. Most enriching strategies are based on chemical modifications, affinity chromatography to capture peptides and proteins containing negatively charged phosphate groups onto a positively charged matrix, or immunoprecipitation by phospho-specific antibodies.

  • New Technological Advancements
  • Olfactory Receptors (CHPP)
  • Other PTMomics & Crosstalks
  • Biocatalysis
  • Molecular engineering
  • Phosphoproteomics, Kinome & PTM Crosstalks
  • Retrometabolic drug design
  • Computational protein engineering
  • Biomimetics

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