Ion Exchange Chromatography

Ion exchange chromatography (IEX) separates molecules present in the mixture based on differences in their net surface charge. Ion exchange chromatography playing an important role in the separation and purification of biomolecules. Today, IEX is one of the most frequently used separation techniques for purification of amino acids, proteins, peptides, and nucleic acids. Ion Exchange Chromatography depends up on charge-charge interactions between the proteins in the sample and the charges immobilized on the resin of choice. Loading samples in buffers of low ionic strength makes ion exchange chromatography an excellent purification step after HIC.

Abstract Submission Registration | Organizing Committee Members | Venue & Hospitality | Previous Speakers

Ion exchange chromatography can be subdivided into Cation exchange chromatography, in which positively charged ions bind to a negatively charged resin; and anion exchange chromatography, in which the binding ions are negative, and the immobilized functional group is positive. Once the solutes are bound, the column is washed to equilibrate it in your starting buffer, which should be of low ionic strength, and then the bound molecules are eluted off using a gradient of a second buffer which steadily increases the ionic strength of the eluent solution.

  • Anion exchange chromatography
  • Cation exchange chromatography
  • Principles involved in Ion exchange chromatography
  • Ion exchange chromatography considerations

Related Conference of Ion Exchange Chromatography

Ion Exchange Chromatography Conference Speakers