Mass Spectrometry Configurations and Techniques

Mass Spectrometry Configurations and Techniques is regards to Mass Spectrometry configuration of source, analyzer, and detector becomes conventional in practice, often a compound acronym arises to designate it, and the compound acronym may be better known among nonspectrometrists than the component acronyms. The Mass Spectrometry instrument consists of three major components those are Ion Source: For producing gaseous ions from the substance being studied; Analyzer: For resolving the ions into their characteristics mass components according to their mass-to-charge ratio and Detector System: For detecting the ions and recording the relative abundance of each of the resolved ionic species. A Imaging Mass Spectrometry is simply a device designed to determine the mass of individual atoms or molecules. Atoms of different elements have different masses and thus knowledge of the molecular mass can very often be translated into knowledge of the chemical species involved. TOF MS is the abbreviation for Time of Flight Mass Spectrometry. Charged ions of various sizes are generated on the sample slide and MALDI is the abbreviation for "Matrix Assisted Laser Desorption/Ionization." Mass spectrometry consists basically of weighing ions in the gas phase. The instrument used could be considered as a sophisticated balance which determines with high precision the masses of individual atoms and molecules. Depending on the samples chemical and mechanical propertiess, different ionization techniques can be used. One of the main factor in choosing which ionization technique to be used is biochemical process. For samples that are not themolabile and relatively volatile, ionization such as Electron Impact and/or Chemical Ionization can be effectively used.

Absolute configuration assignment of isomers such as cis/trans pairs or ortho/meta/para sets based solely on mass spectrometry (MS) data is a very challenging, most often unfeasible task. Configuration assignments are therefore performed indirectly, by comparison of MS data, in an effort that requires access to all isomers within the set and previous configuration assignments by other techniques. Mass spectra are then acquired hoping that each ionized molecule will dissociate to form a unique, structurally diagnostic fragment ion. Otherwise, each neutral or ionized molecule may be found to display characteristic ion-molecule reactivity and to form a unique, structurally diagnostic product ion. When unique ions are not observed, significant variations in product ion ratios from dissociation or bimolecular reactions can still be used for comparative isomer distinction.

  • Instrumentation principles
  • Design and demonstration
  • Mini/Portable/Fieldable mass spectrometry
  • Time-of-flight mass spectrometry
  • Electron transfer dissociation mass spectrometry
  • Separation enhancement by electric means
  • UV and IR spectroscopy
  • Micro/nanostructured materials
  • Solid Phase Micro-Extraction (SPME)
  • Solid liquid separations and purification
  • Liquid-Liquid Extraction

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