Microbial contamination

Microbial Contamination refers to the non-intended or accidental introduction of infectious material like bacteria, yeast, mould, fungi, virus, prions, protozoa or their toxins and by-products. The sources of microbial contamination includes equipment, process operations, raw materials, column resins, filter membranes, water, process gases, and personnel. All sources of microbial contamination should be considered when developing a microbial control strategy and performing an investigation for a microbial contamination deviation. Modern laboratories are busy environments with personnel sharing equipment across overlapping workstations that may be near high-traffic areas and busy instruments.  The prevention of microbial contamination can be done by Practicing good aseptic technique is critical to maintaining the purity of cell cultures as well as a safe lab environment. Some of the most basic laboratory procedures are the most important, including using proper aseptic technique, wearing clean lab coats and washing hands in order to reduce the risk of introducing microorganisms into mammalian cell cultures. Cleanrooms and associated controlled environments provide the control of contamination (inert particles and microbiological entities) to levels appropriate for accomplishing contamination-sensitive activities. Products and processes that benefit from the control of contamination include those in such industries as aerospace, electronics, food and beverages, cosmetics, general healthcare, and medical devices and pharmaceutical products requiring a variety of clean environments.  Environmental Monitoring (EM), particularly in Pharmaceutical manufacturing facilities where the risk of microbial contamination is controlled through aseptic processing, comprises both physical and microbiological test methods

  • Sources of Microbial contamination
  • Prevention of microbial contamination
  • Microbial control in Aseptic processing cleanrooms
  • Microbiologically Controlled Environments in the Pharmaceutical Industry

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