Molecular Exclusion Chromatography

Molecular exclusion also called or named as Size exclusion chromatography (SEC) separates molecules depending on their molecular size by filtration through a gel. The gel consists of spherical beads containing pores of a particular size distribution. Separation occurs when molecules present in the mixture, of different sizes are included or removed from the pores within the matrix. Small molecules and large molecules are separated based on entering in to the pores. Consequently, molecules separate based on their size as they pass through the column and are eluted in order of decreasing molecular weight (MW).

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In molecular exclusion or zize exclusion chromatography, operating conditions and gel selection depend on the application and the desired resolution. Two common types of separations performed by SEC are fractionation and desalting (or buffer exchange.) In molecular exclusion separation mechanism relies solely on the size of the polymer molecules in solution, rather than any chemical nteractions between particles and the stationary phase.

  • Gel filtration chromatography
  • Size exclusion chromatography
  • Principle involved in size exclusion chromatography
  • Applications of molecular exclusion chromatography

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