International Conference on Medical and Clinical Microbiology July 03-04, 2017 AVANI Atrium Bangkok, Bangkok, Thailand

Molecular Techniques

Molecular technique is used in molecular microbiology (Molecular biology is the science of biomolecules) to detect the presence of the pathogens in a sample like air, water, organ tissue, etc.

The application of molecular technology in medicine is almost endless, some of the applications of molecular methods are:

1. Classification of the organism based on genetic relation

2. Identification and confirmation of isolate obtained from the culture

3. Detection of pathogens in clinical specimen

4. Rapid detection of antibiotic resistance

5. Detection of mutations

6. Differentiation of toxigenic  from non-toxigenic strains

7. Detection of microorganisms that lose viability during transport, impossible, dangerous and costly to culture, grow slowly or present in extremely small numbers in the clinical specimen.

 8. Apart from their role in microbiology, these techniques can also be used in identifying abnormalities in forensic medicine and humans.

• Plasmid profiling
• Mol % G+C Contents
• Nucelotide sequencing
• Nucleic acid hybridization
• Pulse field gel Electrophoresis(PFGE)
• Amplification Techniques

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