Partition Chromatography

Partition chromatography is method of separation in which the components present in the mixture get distributed more likely into two liquid phases because of differences in partition coefficients. In Partition chromatography, the molecules are separated in between two phases i.e. both stationary phase and mobile phase are in same phase. So molecules get dispersed into either phase preferentially.  The partition chromatography is the basic principle involved in many separation techniques like high performance liquid chromatography and gas chromatography. Partition chromatography is basically understood as a method of separation of solutes utilizing the partition of the solutes between two liquid phases. Paper chromatography and High performance TLC also works on the principle of the same.

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In partition chromatography molecules get preferential separation in between two phases. i.e. both stationary phase and mobile phase are liquid in nature. So molecules get dispersed into either phases preferentially. Polar molecules get partitioned into polar phase and vice-verse. This mode of partition chromatography applies to Liquid-liquid, liquid-gas chromatography and not to solid-gas chromatography. Because partition is the phenomenon in between a liquid and liquid or liquid and gas or gas and gas. But not in solid involvement.

  • Paper partition chromatography
  • Partition column chromatography
  • Centrifugal partition chromatography
  • Liquid-liquid chromatography
  • Bonded phase chromatography
  • Method development in partition chromatography

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