Quantitative Techniques

Quantitative Techniques is basic practical topic that covers downstream processing techniques suitable for production-scale protein purification and illustrates how these techniques should be considered for process development. The focus of this session is on the understanding of the techniques and the parameters governing separation. Detailed presentations of the downstream processing techniques used (crossflow filtration & chromatography) are included as well as content covering optimization and purification strategies. Scale-up issues, column maintenance, and process hygiene are briefly presented to describe the industrial environment and its principal objectives. Additionally, an overview of analytical techniques used for product identification is presented.

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