Transcriptome Analysis and Gene Expression

For an International conference on Transcriptomics, an overview on Transcriptome analysis and Gene Expression is the first and the essential most topics to be discussed. While going in depth of the subject, it is necessary to understand Transcriptome as Key Players in Gene Expression. For that we should know the basics knowledge of how the central dogma works. This can be achieved by gaining proper knowledge about functioning of mRNA, tRNA and rRNA. Gene expression analysis experiments can focus on a subset of relevant target genes. The location of gene and relative distances between genes on a chromosome can be determined through Sequence mapping. Even in the absence of the reference genome, transcriptome can be created using de novo transcriptome assembly method. Globally around thousands of Universities and institutes are carrying research on gene expression and transcriptome analysis. Being specific are the University of Leeds, Case Western Reserve University, Arizona State University, Tempe. Institutes like The Genome Institute – St. Louis, Missouri and NIH - National Human Genome Research Institute are working tremendously towards the same where researchers are having a database of over 40,000 gene sequences that they can use for this purpose

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The Transcriptome is a complete collection of RNA from an organism, a group of cells, or even a specific cell. Transcription is the  process by which genetic information is copied from DNA to RNA, resulting in a specific protein formation. All together functioning of mRNA, tRNA and rRNA  plays a vital role in making proteins. Mapping and Quantifying Mammalian Transcriptomes provides a digital measure of the presence and prevalence of transcripts from known and previously unknown genes. The sequencing, de novo assembly and annotation of transcriptome datasets generated with next generation sequencing (NGS) has enabled biologists to answer genomic questions in non-model species with unprecedented ease. Genetic regulation of gene expression is the process by which the genetic code - the nucleotide sequence - of a gene is used to direct protein synthesis and produce the structures of the cell. Genes that code for amino acid sequences are known as 'structural genes.Transcriptome profiling reveals many informations about past , present,ongoing and further researches . Genome annotation  bridges the gap from the sequence to the biology of the organism. The aim of high-quality annotation is to identify the key features of the genome — in particular, the genes and their products.

  • Transcriptomes
  • Transcriptome Profiling
  • Functioning of mRNA, tRNA and rRNA
  • Mapping and Quantifying Mammalian Transcriptomes
  • de novo transcriptome assembly
  • Transcription of gene
  • Single-cell Proteins

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Transcriptome Analysis and Gene Expression Conference Speakers