Transcriptome analysis and Gene Expression-An Overview
For an International conference on Transcriptomics, an overview on Transcriptome analysis and Gene Expression is the first and the essential most topic to be discussed. While going in depth of the subject, it is necessary to understand Transcriptome as Key Players in Gene Expression. For that we should know the basics knowledge of how the central dogma works. This can be achieved by gaining proper knowledge about functioning of mRNA, tRNA and rRNA. Gene expression analysis experiments can focus on a subset of relevant target genes. The location of gene and relative distances between genes on a chromosome can be determined through Sequence mapping. Even in the absence of the reference genome, transcriptome can be created using de novo transcriptome assembly method. Globally around thousands of Universities and institutes are carrying research on gene expression and transcriptome analysis. Being specific are the University of Leeds, Case Western Reserve University, Arizona State University, Tempe. Institutes like The Genome Institute – St. Louis, Missouri and NIH - National Human Genome Research Institute are working tremendously towards the same where researchers are having a database of over 40,000 gene sequences that they can use for this purpose.
- Transcriptome as Key Players in Gene Expression
- Functioning of mRNA, tRNA and rRNA
- Sequence mapping
- de novo transcriptome assembly
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