Effect of Lignin Glycosides Extracted from Pine Cones on the Human SIRT1 Promoter

Sirtuins (SIRT1-7) and telomerase (TERT) are known to be involved in the regulation of cellular senescence/ aging processes. Resveratrol (Rsv), a natural polyphenol, which has been shown to extend the lifespan of diverse range of species. Furthermore, Rsv has been shown to activate SIRT1, the class III NAD+ dependent histone deacetylases (HDACs), directly in mammalian cells. Here, we examined the changes in promoter activities of the human SIRT1 and TERT genes after treatment with Rsv and lignin glycosidases/lignin carbohydrate complexes (LCC) extracted from pine cones of Pinus Karalensis. We constructed Luciferase (Luc) expression vectors, pGL4- SIRT1 and pGL4-TERT, containing 396-bp and 263-bp of the 5’-upstream regions of human SIRT1 and TERT genes, respectively, which were then transfected into HeLa S3 cells. The Luc reporter plasmid assay revealed that treatment with the LCC increases not only SIRT1 but also TERT promoter activities more than that with Rsv. These results suggest that the LCC from pine cones induces expression of both SIRT1 and TERT genes, which is thought to have anti-aging effects.


Introduction
It has been suggested that both cellular senescence and aging of organisms are accelerated by various factors, such as telomereshortening [1][2][3] and DNA damaging reactive oxygen species (ROS) that are mainly generated from mitochondria [4,5]. From genetic analyses, it has been shown that several genes, including daf-2 and daf-16, which encode insulin/IGF1 receptor and transcription factor FoxO, respectively, play important roles in controlling the lifespan of C. elegans [6]. Studies of budding yeast showed that Sir2, a member of sirtuins having an NAD + -dependent protein deacetylase, has silencing action on chronological aging of yeast cells [7].
Recently, a natural polyphenolic compound Resveratrol (Rsv), which has a stimulating effect on sirtuin enzyme activity, has been shown to increase the lifespan of model animals [8][9][10][11][12]. Previously, we reported that trans-Resveratrol (Rsv) activates the human TERT promoter thus moderately inducing telomerase activity in HeLa-S3 cells [13]. Moreover, multiple transfection assays showed that promoter activation of the human shelterin protein encoding genes are up-regulated by Rsv treatment [14], suggesting that the natural polyphenol beneficially affects chromosomal stability by trans-activation of telomere maintenance factor encoding genes. Thus, Rsv and its related poly-phenols have been expected to be one of the candidate drugs for anti-aging therapeutics. However, it should be noted that Rsv has cytotoxic effects inducing cell death by apoptosis especially when it is used at high doses [15][16][17]. Therefore, in order to develop safe and effective anti-aging drugs, alternative natural compounds that induce SIRT1 and TERT gene expression should be investigated.
We previously showed that lignins and tannins, which are potential inhibitors of poly (ADP-ribose) glycohydrolase (PARG) [18], have many biological effects as antioxydants, antiviral, anticancer, and immuno-potentiating reagents [19,20]. Furthermore, tannic acid suppressed TPA-induction of HIV promoter activity in Jurkat cells [21,22], suggesting that these natural compounds may affect transcription of various genes. In this study, we examined the effects of lignin carbohydrate complexes (LCC) extracted from pine cones of Pinus Karakensis on the promoter activities of the human TERT and SIRT1 genes by transient transfection and Luc reporter assay. Here, we describe the up-regulating effect of the LCC on these two promoter activities, suggesting that it is an attractive candidate for anti-aging drugs.

Preparation of LCC from Pinus Karalensis pine cones
Our preliminary experiments demonstrated that none of the active material from pine cones of Pinus karalensis was ethanol soluble, all of the following extractions were performed with extensive alcohol washing and acetone drying to remove fatty materials [19,20]. Pine cones thus prepared were extracted with 0.3 N NaOH over night. The pine cone extracts were neutralized by 2 N HCl to pH 5.5, and then an equal volume of ethanol was added. The ethanol precipitates were collected and dialyzed against water. The dialyzates were lyophilized and purified by Sepharose LC-4B and Toyopal HW 40-F column chromatographies. The fractions were evaluated by pGL4-SIRT1 reporter plasmid transfection and Luciferase (Luc) assay, and SIRT1 promoter activating fractions were collected. The partial structures of the purified LCC were analyzed by NMR ( Figure 1). The molecular weight of the LCC is 8,000 to 10,000 containing uronic acid (10 to 20% of total sugar residues) and neutral sugars, including glucose, galactose, mannose, and arabinose (80 to 90% of total sugar residues).

Transient transfection and luc assay
Plasmid DNAs were transfected into HeLa S3 cells by the DEAEdextran method [22,23]. The DNA-transfected cells were divided into at least four dishes. After 24 h of transfection, pine cone LCC, trans-Resveratrol (Cayman Chem., Ann Arbor, MI) or other natural compounds were added to the culture medium. After a further 24 h of incubation, cells were collected and lysed with 100 µL of 1 X cell culture lysis reagent, containing 25 mM Tris-phospate (pH 7.8), 2 mM DTT, 2 mM 1,2-diaminocyclohexane-N,N,N',N',-tetraacetic acid, 10% glycerol, and 1% Triton X-100, then mixed and centrifuged at 12,000 x g for 5 sec. The supernatant was stored at -80°C. The Luc assay was performed with a Luciferase assay system (Promega) and relative Luc activities were calculated as described previously [22,23].

Isolation and characterization of the 5'-flanking region of the human SIRT1 gene
The nucleotide sequence contained in the pGL4-SIRT1 revealed that the 0.4-kb region is identical to that of GenBank Accession No.

Effect of LCC on the SIRT1 promoter
To examine whether human SIRT1 promoter is affected by natural compounds, including LCC obtained from pine cone extracts ( Figure  1), transient transfection and Luc assay were carried out. Luc activities of pGL4-SIRT1 transfected cells were normalized to that of non-treated control cells. As shown in Figure 3A left panel, relative Luc activity of the pGL4-SIRT1-transfected cells was prominently augmented by the addition of Rsv (10 µM) or LCC (100 µg/ml) to the culture medium. In contrast, 100 µM of Quercetin, which is a natural polyphenolic flavonoid [25], moderately up-regulated the SIRT1 promoter activity ( Figure 3A, left).
To examine the dose-dependent response of LCC, HeLa S3 cells were treated with 0 to 100 µg/mL of LCC after 24 h of transfection and collected after further 24 h incubation ( Figure 3B, open circle). The results indicated that 10 µg/ml of LCC is enough to induce SIRT1 promoter activity equal to Rsv (10 µM) treatment. The half maximal effective concentration (EC 50 ) was calculated to be 18 µg/ml. The endotoxin concentration in the LCC was estimated to be 15.7 EU/mg, suggesting that it contains LPS only at low levels.

Effect of LCC on the TERT promoter
Similar experiments with pGL4-TERT plasmid were performed ( Figure 3A, right). The results indicated that the 263-bp of the human TERT promoter region responded to Quercetin (100 µM), Rsv (10 µM), and LCC (100 µg/ml) in a similar manner as the 396-bp of the SIRT1 promoter ( Figure 3A, left). In addition, the LCC-dose response of the TERT promoter was almost the same as that of the SIRT1 promoter ( Figure 3B).

Discussion
In this study, we have isolated a 5'-flanking region of the human SIRT1 gene, and observed that Luc activity under the control of this 396-bp region showed greater response to the treatment with LCC  than that with Rsv or Quercetin in HeLa S3 cells. This region has no apparent TATA-box but contains several well-known transcription factor binding sequences, including CREB, C/EBPβ, c-ETS, USF, SREBP1, Sp1, GATA, and c-MYC binding elements (Figure 2). At present, the LCC-and Rsv-responsive elements in the SIRT1 promoter region have not been precisely determined. However, the 5'-upstream regions of the WRN, BLM, TERT, p21 (CDKN1A), and HELB genes, in which one or more Sp1/GC-box elements are commonly located, positively respond to the Rsv treatment in HeLa S3 cells [13,26]. The GC-box consensus sequence of the Sp1 transcription factor binding site is: 5'-(G/T)GGGCGG(G/A)(G/A)(C/T)-3' or 5'-(G/T)(G/A) GGCG(G/T)(G/A)(G/A)(C/T)-3' [27]. It has been shown that two GC-boxes, 5'-AGGGCGGGGG-3' and 5'GGGGCGGGTC -3' (Figure  2, -83 to -74 and -66 to -57, respectively), play important roles in the SIRT1 promoter activity [28]. Therefore, the tandem repeat of the Sp1 binding motif is possibly one of the candidate elements that respond to the LCC treatment. In addition, Rsv up-regulates cAMP level and CREB is known to be regulated by cAMP, and plays important roles in hormonal metabolisms, including that of the insulin signaling system [29]. These observations suggest that the CREB element located in the human SIRT1 promoter region (-288 to -281) may be also involved in the response to the LCC and Rsv treatments.
Rsv has been shown to affect essential signal transduction factors, including AMPK [30,31] and FoxO [32], directly regulating the deacetylase activity of SIRT1. Other polyphenols, such as Quercetin and Catenins have been reported to moderately activate mammalian SIRT1 or yeast Sir2 enzyme activity [10,25,33]. However, they have no such effect in cellular system [25,33], rather they inhibit SIRT1 activity [33]. Therefore, regulation of lifespan and longevity of organisms might not only be controlled by SIRT1 enzyme activity but also by other factors involved in signal pathways between Rsv and SIRT1. Recently, it was suggested that Rsv has a hormetic effect on cells or organisms, that is to cause a biphasic dose response of various biological events [34]. Here, for the first time, we found the effective up-regulation of the SIRT1 and TERT gene promoter activities by the LCC treatment, suggesting that regulation of some transcription factors, including Sp1, responsible for the LCC may play important roles in control for life spans. Furthermore, the core structures of LCC might be useful to generate lead compounds for novel anti-aging drugs, which could simultaneously activate the SIRT1 and TERT promoter activities.