|Recombinant protein purification was performed by metal chelate affinity chromatography using the His-BindTM purification kit according to manufacturerâs instructions (Novagen), and chromatography samples were analysed by SDS PAGE and chemiluminescent western immunoblotting as before. Pre-operative vaccination in elective orthopaedic cases could be a viable alternative approach for prevention of infection to reduce both infection rate and severity. There are increasing requirements to develop complimentary preventive strategies without giving rise to antibiotic resistance or undesirable implant/fixative effects on integration. A vaccine against staphylococcal biomaterial-related infection will likely target multiple surface expressed virulence factors from S. aureus and S. epidermidis (significant causative organism) that are crucial to bacterial adherence and biofilm formation.
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