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Wolbachia are intracellular bacteria that are required for development and reproduction in most filarial nematodes. New approaches are needed to improve understanding of the role of Wolbachia in filarial biology. Recent studies using fluorescent immunohistology revealed asymmetric segregation of Wolbachia in early embryos of Brugia malayi, and this helps to explain the restricted distribution of Wolbachia in adult worms. However, the distribution of Wolbachia in late stages of embryo development or in infective larvae (L3) is largely unknown. In this study we have explored the use of in situ hybridization (ISH) to localize Wolbachia and Wolbachia gene expression during embryogenesis and morphogenesis in B. malayi. Daojun Jiang, Use of in situ Hybridization to Localize Wolbachia during Embryogenesis in Brugia malayi
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Last date updated on April, 2023