alexa Immunogenicity and Safety of an Inactivated Hepatitis A Vaccine Given with Measles-Mumps-Rubella Vaccine to 12-13 Month Old Turkish Children | OMICS International
ISSN: 2157-7560
Journal of Vaccines & Vaccination

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Immunogenicity and Safety of an Inactivated Hepatitis A Vaccine Given with Measles-Mumps-Rubella Vaccine to 12-13 Month Old Turkish Children

Kadriye YURDAKÖK1*, Mustafa BAKIR2, Tolga INCE1, Songül YALÇIN1, Elif ÖZMERT1, Ahmet SOYSAL2, Tamer PEHLIVAN 3 and Anvar RASULI4

1Hacettepe University Faculty of Medicine, Department of Pediatrics,Social Pediatrics Unit, Ankara, Turkey

2Marmara University Hospital, Department of Pediatrics, Division of Pediatric Infectious Diseases, Istanbul, Turkey

3Sanofi Pasteur, Istanbul, Turkey

4Sanofi Pasteur, Lyon, France

*Corresponding Author:
Pediatric Health and Diseases Department
Social Pediatrics Unit
Hacettepe University Faculty of Medicine, Ankara, Turkey
Tel: +90 312 305 11 33
Fax: +90 312 324 32 84
E-mail: [email protected]

Received date: September 10, 2012; Accepted date: September 20, 2012; Published date: September 25, 2012

Citation: YURDAKÖK K, BAKIR M, SOYSAL A, İNCE T, YALÇIN S, et al. (2012) Immunogenicity and Safety of an Inactivated Hepatitis A Vaccine Given with Measles-Mumps-Rubella Vaccine to 12-13 Month Old Turkish Children. J Vaccines Vaccin 3:146. doi:10.4172/2157-7560.1000146

Copyright: © 2012 YURDAKÖK K, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Background: The epidemiology of hepatitis A in Turkey favor routine vaccination of children. This study assessed compatibility of a monovalent hepatitis A vaccine with the measles-mumps-rubella (MMR) combination vaccine used in the national immunization calendar at 12-15 months of age. Methods: Hepatitis A seronegative participants were randomized equally to hepatitis A vaccine followed by MMR vaccine 28 days later (Group A), MMR vaccine followed by hepatitis A vaccine 28 days later (Group B), or one dose each of hepatitis A and MMR vaccines given on Day 0 (Group C). All participants received a hepatitis A booster dose 6 months later. Results: A total of 470 seronegative (anti-hepatitis A concentration ≥20 mIU/mL) participants were randomized: 188 to Group A, 94 to Group B and 188 to Group C. The hepatitis A seroprotection rates (≥20 mIU/mL), 1 month after the first vaccination were 93.6% (Group A) and 92.7% (Group C) by a microparticle enzyme immunoassay (MEIA). Using a more sensitive electrochemiluminescence immunoassay (ECLIA), the corresponding seropositivity rates were 100 and 99.4%. Non-inferiority of seroprotection against hepatitis A was demonstrated for concomitant versus non-concomitant vaccination using ECLIA, but not MEIA. After booster, all participants had anti-hepatitis titers ≥20 mIU/mL and anti-hepatitis geometric mean concentrations of 5,078 mIU/mL (Group A), 3,271 mIU/mL (Group B) and 4,314 mIU/mL (Group C). Following primary vaccination, measles (≥120 mIU/mL) and mumps (≥ 10 AU/mL) seroprotection rates were 96.5% with both separate and concomitant vaccination. The rubella seroprotection (≥ 10 AU/mL) rates were 97.6 and 96.7% following separate and concomitant vaccination, respectively. Reactogenicity increased slightly with concomitant administration; both vaccines were well tolerated. Conclusions: The immune response to the hepatitis A vaccine was not impaired by concomitant administration with MMR vaccine. Non-inferiority of seroprotection was demonstrated with the more sensitive of two assays used to evaluate anti-hepatitis A antibody.


Hepatitis A vaccine; Co-administration; Pediatric vaccines; Measles, Mumps rubella vaccine


In 2005, following a successful vaccination campaign to reduce the incidence of measles in Turkey, the National Immunization Program (NIP) replaced monovalent measles vaccination at 9 months of age with combination measles, mumps, rubella (MMR) combination vaccine given at 12 months and at 6 years of age [1].

The epidemiology and burden of hepatitis A also favors routine universal vaccination of children in Turkey. The World Health organization (WHO) classifies hepatitis A virus (HAV) endemicity as high, intermediate or low, depending on the prevalence of anti-HAV (IgG) antibodies in sera of specific age groups [2]. Improved hygiene and sanitation result in an overall reduction in HAV circulation and decrease of endemicity from high to intermediate and from intermediate to low. Epidemiologic monitoring and accurate surveillance of vaccinepreventable diseases play an important role in the selection of vaccines for inclusion in the NIP and the vaccination strategies that are followed. Evidence of decreasing HAV seroprevalence in younger age groups are over more than a decade has been reported in Turkey [3-5]. People who are highly susceptible - because of decreased seroprevalence - may live together with people among whom the HAV widely circulates. Consequently, many individuals escape early childhood infection and are infected later in life when they are more likely to develop severe hepatitis A disease. A survey of HAV seroprevalence in Turkey demonstrated clear differences among five regions, with seropositivity in children between 5-9 years of age >80% in the south-east and east, and <50% in central and western regions [6]. Hepatitis A is a reportable disease in Turkey. Hepatitis A incidence in the Izmir region in 2003 was estimated at 27.66/100,000 from case and laboratory reports or 53 cases/100,000 by the capture-recapture method [7]. Seroprevalence and surveillance data in Turkey indicate intermediate endemicity, supporting routine childhood hepatitis A vaccination.

As with MMR, the first dose of hepatitis A vaccine is generally given at 12 months of age. Giving both vaccines at the same time would avoid separate clinic visits and would potentially assure the new vaccine would have coverage as high as the existing MMR vaccine. Before adding hepatitis A vaccination to the NIP, the safety and immunogenicity of the vaccine given concomitantly with MMR vaccine at different injection sites must be documented. Previous studies have shown hepatitis A and MMR vaccines were compatible when given concomitantly, but the data is limited [8-10]. Therefore, this open, randomized, controlled study was conducted to assess whether hepatitis A and MMR vaccination (Avaxim™ 80U Pediatric and Trimovax™, Sanofi Pasteur, Lyon, France) could be administered concomitantly at 12 to 15 months of age to children.

Materials and Methods

Trial design and participants

This Phase IV, observer-blind trial ( NCT00313950) was conducted at two centers in Turkey. The Ethics Committee/Institutional Review Board at each study center approved the protocol, written informed consent forms, recruitment procedures and other written information provided to participants. The trial was conducted in accordance with the Helsinki Declaration, International Conference on Harmonization and Good Clinical Practice guidelines. The participant’s parent(s)/legal representative gave written informed consent before enrolment.

Healthy children, 12-13 months of age, born at full term pregnancy (>37 weeks) with a birth weight of ≥2.5 kg, were eligible if they were hepatitis A seronegative. Receipt of one dose of monovalent measles vaccine at 9 months of age and before enrollment was allowed. Participants were excluded if they were hepatitis A seropositive, were enrolled in another clinical trial during the 4 weeks prior to first study vaccination, or planned to participate in another clinical trial during the course of this trial; had congenital or acquired immunodeficiency; received immunosuppressive therapy such as long-term systemic corticosteroids therapy; were allergic to any study vaccine component; had a chronic illness that could interfere with trial conduct or completion; had received blood or blood-derived products in the past 3 months; had any vaccination in the past 4 weeks, or planned in the 4 weeks following the first trial vaccination; history of (confirmed) hepatitis A, measles, mumps, and/or rubella infection; previous hepatitis A or MMR vaccination; thrombocytopenia or a bleeding disorder contraindicating intramuscular (IM) injections; history of seizures; or a febrile illness (axillary temperature ≥37.4°C) on the day of enrolment.

Participants were screened for hepatitis A seropositivity after they gave informed consent. Seronegative participants were randomly assigned equally to one of three study groups: Hepatitis A vaccine followed by MMR 28 days later (Group A), MMR vaccine followed by hepatitis A vaccine 28 days later (Group B), or one dose of hepatitis A vaccine and one dose of MMR given at two different sites on Day 0 and Day 28 (Group C). All participants received a hepatitis A vaccine booster dose 6 months after the first dose. Each participant was followed-up for approximately 8 months.

Group assignment was performed using a list of random numbers supplied by the study sponsor. As both vaccines were commercially available and easily identifiable, the study design was observer-blind, with only the personnel who administered the vaccines being aware of the study group assignments. The investigators, parent(s)/guardian(s) did not witness administration.


Avaxim™ 80U Pediatric is an inactivated hepatitis A vaccine containing 80 antigen units of formaldehyde-inactivated GBM strain hepatitis A virus in each 0.5 mL dose. Trimovax™ is a trivalent MMR vaccine containing ≥ 1,000 tissue culture infective dose 50% (TCID50) Schwarz strain measles, ≥ 5,000 TCID50 Urabe Am9 strain mumps, and ≥ 1,000 TCID50 Wistar RA 27/3M rubella live attenuated viruses in each 0.5 mL dose. Both vaccines are licensed in Turkey and were provided by Sanofi Pasteur, Lyon, France. The hepatitis A vaccine (commercial batches Y1063-1, A1182-2 and Z0532-1) was supplied as a liquid suspension in single-dose prefilled syringes. The MMR vaccine (batches Z5334-2, Z6638-5, A5715-1 and B5576-4) was supplied as a freeze-dried powder in single-dose vials and reconstituted with water for injection. Both vaccines were administered by IM injection into the deltoid muscle.

Laboratory methods

Four blood samples were collected: 7 days prior to enrolment in order to determine hepatitis A sero-status and for measurement of prevaccination antibody concentrations, at 4 weeks (Day 28), at 7 months after the first vaccination (Day 213), and 4 weeks after hepatitis A booster vaccination (Day 243).

Anti-Hepatitis A antibody assessment: Anti-hepatitis A antibody concentrations at enrollment, after primary vaccination, before and after booster vaccination were measured at BARC laboratories in Ghent, Belgium by using a commercially available microparticle enzyme immunoassay (MEIA) (Abbott Axsym HAVAB 2.0, Abbott Park, Illinois, USA). The lower limit of quantification (LLOQ) for that assay was 5 mIU/mL. The cut off value for HAV seropositivity was defined as 20 mIU/mL; participants with concentrations <20 mIU/mL at screening were considered seronegative and eligible for the trial. As no absolute threshold of hepatitis A seroprotection has been identified [11], both 5 mIU/mL and 20 mIU/mL were used in the immunogenicity analysis.

Additional serological testing was performed at the same laboratory using a commercially available electrochemiluminescence immunoassay (ECLIA) (Roche Elecsys Anti-HAV, Roche Diagnostics, Indianapolis, USA) to measure hepatitis A IgM and IgG in postscreening sera from the enrolled subjects, including post first-dose sera from Groups A and C on Day 28, and pre-booster samples from all three study groups. The LLOQ for the assay was 3 mIU/mL. The ECLIA test results were analyzed following the statistical plan for Group A and Group C seroprotection rates after the first vaccine dose and prior to administration of the booster dose. With the ECLIA test, both ≥5 mIU/ mL and ≥20 mIU/mL thresholds were considered for the hepatitis A seroprotection rate.

Anti-measles, mumps and rubella antibody assessment: Anti- Measles, mumps and rubella antibody concentrations at enrollment and 1 month after vaccination were measured by enzyme-linked immunosorbent assay (ELISA) at PPD Laboratories in Wayne, PA, USA. The LLOQs for the assays were 60 mIU/mL for measles, 5 antigen units (AU) per mL for mumps and 5 IU/mL for rubella. The cut off values for seropositivity/seroprotection were defined as 120 mIU/mL for measles, 10 mIU/ml for rubella, and 10 AU/mLfor mumps.


Subjects were monitored for immediate reactions in the first 30 minutes following each vaccine injection. Parents/guardians recorded the occurrence, intensity and duration of solicited injection site (tenderness, redness, swelling) and systemic (fever, vomiting, abnormal crying, drowsiness, appetite lost, irritability) reactions to occur on the day of vaccination and for 7 days afterwards. Unsolicited and serious adverse events (SAEs) were recorded during the whole study period. Information was collected in the source document and in the CRF for each subject.

Statistical methods

Seroprotection rates (SP) against hepatitis A at 4 weeks (Day 28) were assessed for non-inferiority of the concomitant administration of HAV and MMR vaccines (Group C) compared with administration of HAV and MMR on different days (Group A) using the 95% confidence intervals (CI) of the SP difference and a clinically acceptable noninferiority limit of 5%. To demonstrate non-inferiority, the 95% CI lower limit for the difference in SP rates between concomitant and separate vaccination had to be greater than -5%.

The sample size calculation indicated 170 participants would provide a power of 90% to demonstrate non-inferiority assuming a hepatitis A seroprotection (≥20 mIU/mL) rate of 99% and a clinically acceptable non-inferiority limit of 5%. We decided to enroll 190 subjects in Groups A and C assuming a dropout rate of 10%. A sample size of 90 participants was considered adequate for descriptive evaluation of immunogenicity in Group B.

In each study group, geometric mean concentrations (GMCs) with corresponding 95% CIs and the seropositivity/seroprotection rates with 95% CIs for each of the vaccine antigens were calculated. Post- to pre-booster GMC ratios (GMCRs, geometric means of individual anti- HAV concentration ratios) were also calculated with their 95% CIs). All immunogenicity analyses were performed according to per-protocol and with the intent-to-treat (ITT) populations. Descriptive statistics were used for analysis of safety data.


Subject disposition

A total of 654 participants were screened between 28 September 2006 and 06 February 2009. Of these, 470 were HAV seronegative (anti- HAV concentration ≥20 mIU/mL) and were randomized to three study groups: 188 to Group A (hepatitis A vaccine on Day 0 + MMR on Day 28): 94 to Group B (MMR on Day 0 + hepatitis A vaccine on Day 28) and 188 to Group C (hepatitis A vaccine and MMR on Day 0). The three study groups were similar to each other with respect to gender and age of participants at enrollment; 55.3 to 57.4% of subjects were male and mean age was12.3 months (range, 11.6-13.3 months).

Of the 470 randomized participants, 436 (92.8%) completed the primary phase and 398 (91.3%) completed the booster phase. Figure 1 summarizes the disposition of participants. Two participants had an AE that led to premature discontinuation. One had acute gastroenteritis, which was judged unrelated to vaccination; the second had a grade 3 fever following vaccination and was withdrawn by the parents. The relation of fever to vaccination was not determined. No early withdrawal resulted from a SAE considered to be related to vaccine administration.


Figure 1: Participant disposition.

All vaccinated participants were included in the safety evaluation. The intent to treat analysis set for evaluation of primary vaccination at Day 28 consisted of all vaccinated participants with an evaluable blood sample. The per-protocol set consisted of 91.5% of participants in Group A, 90.4% in Group B and 87.2% in Group C. The most common reason for exclusion from the per-protocol analysis was absence of blood drawn on Day 28 followed by a too long interval between vaccination and blood sample collection exceeding the permissible 25- 60 days. The post-booster immunogenicity analysis set included 81.9% of the participants in Group A, 85.1% in Group B, and 78.7% in Group C. The most common reasons for exclusion were absence of booster vaccination and absence of a post-booster blood sample.


Hepatitis A: The seroprotection rates and GMCs for both hepatitis A and MMR vaccines are summarized in Tables 1 and 2. The hepatitis A seroprotection rates (≥20 mIU/mL, MEIA assay) one month after the first vaccination were 93.6% (161/172 participants) and 92.7% (152/164 participants) in Groups A and C, respectively. The difference in SP rates for Group C vs. Group A was -0.92% (95% CI: [-6.68; 4.69]). As the lower limit of the 95% CI was <5%, seroprotection following concomitant administration of the hepatitis A and MMR vaccines was not non-inferior to hepatitis A vaccine administered alone. Noninferiority of seroprotection (≥20 mIU/mL) against hepatitis A was demonstrated using the more sensitive ECLIA assay, which resulted in SP rates of 100% and 99.39% for non-concomitant and concomitant administration, respectively (Table 3). With a seroprotection threshold of ≥5 mIU/mL, non-inferiority of concomitant vaccination was demonstrated by both the MEIA and ECLIA assays.

Group A: HAV vaccine Day 7 & MMR Vaccine Day 28 Group B: MMR vaccine Day7 & Hav vaccine Day 28 Group C: HAV vaccine & MMR vaccine Day 0
Day -7 Day 28 Day 213 Day 243 Day -7 Day 28 Day 213 Day 243 Day -7 Day 28 Day 213 Day 243
N Data† 172 172 154 154 85 84 79 80 164 164 148 148
GMC (mIU/mL) 5.01 46.78 217.11 5078.74 5.06 3.67 97.92 3271.00 5.40 42.42 144.06 4314.86
[95% CI] [4.54;5.53] [42.43;51.58] [191;246] [4433;5818] [4.38;5.85] [3.28;4.11] [78.58;122.02] [2657.9;4025.6] [4.83;6.04] [38.86;46.30] [127.0;163.4] [3757.76;4954.54]
≥5 mIU/mL
% (n)

59.3 (102)

98.3 (169)

100.0 (154)

100.0 (154)

60.0 (51)

39.3 (33)

100.0 (79)

100.0 (80)

61.6 (101)

100.0 (164)

100.0 (148)

100.0 (148)
[95% CI] [50.87;65.44] [94.99;99.64] [97.6;100.0] [97.63;100.0] [48.80;70.48] [28.80;50.55] [95.44;100.0] [95.49;100.0] [53.68;69.06] [97.78;100.0] [97.54;100.0] [97.54;100.0]
≥20 mIU/mL
% (n)

1.2 (2)

93.6 (161)

100.0 (154)

100.0 (154)

1.2 (1)

1.2 (1)

94.9 (75)

100.0 (80)

3.6 (6)

92.7 (152)

99.3 (147)

100.0 (148)
[95% CI] [0.14;4.14] [89.2;96.8] [97.6;100.0] [97.6;100.0] [0.03;6.39] [0.03;6.46] [87.54;98.60] [95.49;100.0] [1.35;7.79] [87.57;96.1] [96.3;99.9] [97.54;100.0]
GMCR NA 9.34 NA 23.39 NA NA NA 33.50 NA 7.85 NA 29.95
[95% CI] [8.07;10.79] [20.7;26.4] [28.36;39.58] [6.84;9.01] [26.35;34.05]

Table 1: Anti-hepatitis A GMC, GMCR and seroprotection rates by micro enzyme immunosorbent assay (MEIA) at enrollment, one month after primary vaccination (Day 28), at booster administration (Day 213) and 1 month after booster administration (Day 243).

  Group A* Group B* Group C*
Day -7 Day 28 Day -7 Day 28 Day -7 Day 28
N 186 178 92 85 186 173
Measles mIU/mL
GMT   221.12 2074.24 2103.77
[95% CI] [1.29;37928.87] [1745.06;2465.52] [1856.55;2383.90]
≥120 mIU/mL: % (n)
2.7 (5)

1.1 (2)

1.1 (1)

96.5 (82)

2.7 (5)

96.5 (167)
[95% CI] [0.88;6.16] [0.14;4.00] [0.03;5.91] [90.03;99.27] [0.88;6.16] [92.60;98.72]
Mumps (AU/mL)
GMT 24.91 61.30 64.92
[95% CI] [4.58;135.45] [52.48;71.61] [58.52;72.03]
≥10 AU/mL: % (n)
4.8 (9)

2.2 (4)

7.6 (7)

96.5 (82)

4.3 (8)

96.5 (167)
[95% CI] [2.24;8.99] [0.62;5.65] [3.11;15.05] [90.03;99.27] [1.87; 8.30] [92.60;98.72]
Rubella (IU/mL)
GMT 44.40 92.85 96.42
[95% CI] [75.75;113.82] [83.91;110.79]
≥10 IU/mL:
% (n)

0.5 (1)

0.6 (1)

0.0 (0)

97.6 (83)

0.5 (1)

96.5 (167)
[95% CI] [0.01;2.96] [0.01;3.09] [0.00;3.93] [91.76;99.71] [0.01;2.96] [92.60;98.72]

Table 2: Anti-measles, anti-mumps and anti rubella GMC and seroprotection rates at enrollment and 1 month (DAY 28) after vaccination.

Group A* Group C* Group C - Group A
n/N % [95% CI} n/N % [95% CI} Difference [95% CI]
Anti-HAV 1
≥20 mIU/mL
161/172 93.60 [88.85; 96.76] 152/164 92.68 [87.57; 96.16] -0.92 [-6.68;4.69]
Anti-HAV 1
≥5 mIU/mL
169/172 98.3 [94.99; 99.64] 164/164 100 [97.78; 100.00] 1.74 [00.82;5]*
Anti-HAV 2
≥20 mIU/mL
169/169 100.0 [97.7; 100.0] 162/163 99.39 [96.63; 99.98]; -0.61 [-3.39;1.67]*
Anti-HAV 2
≥5 mIU/mL
172/172 100 [97.84; 100.0] 163/164 100 [97.76; 100] -0.58 [-3.33; 1.62]*

Table 3: Anti-HAV Seroprotection Rate one month after primary vaccination (Day 28).

Before primary vaccination (Day 7), more than half of the participants had anti-HAV with concentrations ≥5 mIU/mL. After a month this percentage decreased from 59.6 to 39.3% and the GMC decreased from 5.12 to 3.67 mIU/mL in Group B between enrollment and hepatitis A vaccination, suggesting that the anti-HAV antibodies present before vaccination were most likely of maternal origin.

Anti-HAV GMCs (MEIA assay) were low in all groups before primary vaccination, but reached 46.52 mIU/mL in Group A and 43.15 mIU/mL in Group C 1 month following primary vaccination; post- to pre-booster GMCRs were 9.43 in Group A and 7.99 in Group C. At 6 months after primary vaccination (Day 213) all participants had anti- HAV concentrations ≥5 mIU/mL; 100% of those in Group A, 94.9% in Group B, and 99.3% in Group C had anti-HAV titers ≥20 mIU/mL. GMCs were higher at 6 months than GMCs at 1 month after priming in all groups, ranging from 97.92 mIU/mL (Group B) to 217.11 mIU/ mL (Group A). Anti-HAV concentrations increased significantly in all groups at 1 month after booster administration (Day 243). All participants had anti-HAV titers ≥20 mIU/mL. GMCs ranged from 3,271.00 mIU/mL (Group B) to 5,078.74 mIU/mL (Group A). Postto pre-booster GMCRs were 23.39 in Group A, 33.50 in Group B, and 29.95 in Group C.

Anti-HAV antibody titers were higher at each time point when measured by ECLIA compared to MEIA. One month after primary vaccination (Day 28), GMCs were 538.41 mIU/mL and 339.54 mIU/mL in Group A and Group C respectively. In contrast to results obtained by the MEIA assay, GMTs measured by ECLIA decreased between primary and booster vaccination, to 315.83 and 205.19 mIU/mL in groups A and C, respectively. Before the booster dose (Day 213), 100% of Group A, 94.9% of Group B, and 99.3% of group C participants had anti-HAV concentrations ≥20 mIU/mL, and 100% of participants had concentrations ≥5 mIU/mL.

Measles, mumps, and rubella: At enrollment, seroprotection rates (Table 2) were low, from 1.1% to 2.7% for measles (≥120 mIU/mL), 4.3% to 7.6% for mumps (≥10 AU/mL) and 0.0% to 0.5% for rubella (≥10 IU/mL). None of the participants in the per protocol analysis set had received monovalent measles vaccine before study enrolment. Following primary vaccination, measles and mumps seroprotection rates were 96.5% with both separate (Group B) and concomitant (Group C) vaccination. The rubella seroprotection rates were 97.6 and 96.7% following separate and concomitant vaccination, respectively. Similar, strong increases in anti-measles, -mumps and -rubella GMC were observed in response to vaccination with both regimens (Table 2).


Most solicited reactions after primary vaccination occurred in the first 4 days, lasted less than 3 days and were of grade 1 or grade 2 intensity. Approximately 62% of participants in Groups A and B and 71% in Group C reported solicited reactions following the first dose of hepatitis A or MMR vaccine (Table 4). The most frequently reported injection site reaction was tenderness, reported by 22-27% of participants in each group. In Groups A, B and C, occurrence of systemic reactions after the first dose of hepatitis A or MMR vaccine was 54.9%, 58.0%, and 64% respectively. The most common systemic reactions were irritability, abnormal crying and loss of appetite. Only 2 participants (1.1%) in Group A and 3 (1.7%) in Group C reported Grade 3 injection site reactions after hepatitis A vaccination. Two participants in Group C reported grade 3 reactions after MMR vaccination. Grade 3 systemic solicited reactions were reported by 18 participants (9.9%) in Group A, 14 (15.9%) in Group B and 22 (11.9%) in Group C.

Group A* (N= 188) Group B* (N= 94) Group C* (N= 188)
Severity n/N % [95% CI] n/N % [95% CI] n/N % [95% CI]
Injection site reactions
Tenderness Any 40/182 22.0 [16.2;28.7] 20/88 22.7 [14.5;32.9] 48/177 27.1 [20.7;34.3]
Grade 3 2/182 1.1 [0.1;3.9] 0/88 0.0 [0.0;4.1] 4/177 2.3 [0.6;5.7]
Erythema Any 14/182 7.7 [4.3;12.6] 7/88 8.0 [3.3;15.7] 26/177 14.7 [9.8;20.8]
Grade 3 0/182 0.0 [0.0;2.0] 0/88 0.0 [0.0;4.1] 0/177 0.0 [0.0;2.1]
Swelling Any 10/182 5.5 [2.7;9.9] 5/88 5.7 [1.9;12.8] 15/177 8.5 [4.8;13.6]
Grade 3 0/182 0.0 [0.0;2.0] 0/88 0.0 [0.0;4.1] 0/177 0.0 [0.0;2.1]
Systemic reactions
Fever Any 11/182 6.0 [3.1;10.6] 8/88 9.1 [4.0;17.1] 22/177 12.4 [8.0;18.2]
Grade 3 0/182 0.0 [0.0;2.0] 1/88 1.1 [0.0;6.2] 0/177 0.0 [0.0;2.1]
Vomiting Any 31/182 17.0 [11.9;23.3] 19/88 21.6 [13.5;31.6] 42/177 23.7 [17.7;30.7]
Grade 3 1/182 0.5 [0.0;3.0] 1/88 1.1 [0.0;6.2] 2/177 1.1 [0.1;4.0]
Abnormal crying Any 47/182 25.8 [19.6;32.8] 30/88 34.1 [24.3;45.0] 58/177 32.8 [25.9;40.2]
Grade 3 4/182 2.2 [0.6;5.5] 0/88 0.0 [0.0;4.1] 6/177 3.4 [1.3;7.2]
Drowsiness Any 24/182 13.2 [8.6;19.0] 14/88 15.9 [9.0;25.2] 36/177 20.3 [14.7;27.0]
Grade 3 0/182 0.0 [0.0;2.0] 0/88 0.0 [0.0;4.1] 0/177 0.0 [0.0;2.1]
Appetite lost Any 62/182 34.1 [27.2;41.4] 28/88 31.8 [22.3;42.6] 73/177 41.2 [33.9;48.9]
Grade 3 7/182 3.8 [1.6;7.8] 9/88 10.2 [4.8;18.5] 12/177 6.8 [3.6;11.5]
Irritability Any 83/182 45.6 [38.2;53.1] 47/88 53.4 [42.5;64.1] 91/177 51.4 [43.8;59.0]
Grade 3 9/182 4.9 [2.3;9.2] 7/88 8.0 [3.3;15.7] 14/177 7.9 [4.4;12.9]

Table 4: Summary of solicited reactions within 8 days after primary hepatitis A and MMR vaccination.

Solicited reactions were slightly less frequent following the hepatitis A booster compared to primary vaccination. Following the HAV booster injection on Day 213, 44.0% to 46.9% of participants reported a solicited reaction; 22.5% to 26% percent experienced an injection site reaction, and 32.8% to 37.9% experienced a systemic reaction. From 2.2% to 4.5% reported Grade 3 injection site reactions and 5.7% to 9.6% reported grade 3 systemic reactions.

Overall, 0.5% of the subjects in Group A, 2.1% of the subjects in Group B, and 1.1% of the subjects from Group C reported at least one unsolicited reaction. One participant in Group A experienced a rash on the head and neck after hepatitis A vaccination. In Group B, one participant experienced a systemic macro-papular rash and another experienced injection site bruising. One participant in Group C experienced injection site ecchymosis and another experienced a macro-papular rash. All were of grade 1 intensity.

Unsolicited adverse events (AEs) were reported by 21.8% to 27.1% of each study group between enrollment and Day 28. The vast majority were grade 1 or grade 2 systemic events that were childhood infections and considered to be unrelated to vaccine injection(s). Three Grade 3 unsolicited AEs were reported in 3 Group A participants, diagnosed as pneumonia and bronchiolitis requiring hospitalization in two participants and acute gastroenteritis in another, all were considered to be Systemic AEs (SAEs). A grade 3 event, bronchiolitis in a Group C participant also required hospitalization and was considered as SAE. After the booster vaccination, 11.7% of the participants in Group A, 9.6% in Group B, and 8.0% in Group C experienced at least one unsolicited AE. All were of grade 1 or grade 2 intensity and all but one (a case of nasopharyngitis) were considered to be unrelated to vaccine injection. In all groups, the most common unsolicited systemic AEs were childhood infections.

Six participants experienced a total of 7 SAEs. Four were reported between enrollment and Day 28 (1 case of pneumonia and 1 case of bronchiolitis in Group A; 1 case of bronchopneumonia and 1 case of bronchiolitis in Group C). Three SAEs occurred between Day 28 and booster vaccination (1 case of cow milk allergy, and 2 cases of laryngitis). No SAE was considered by the investigator to be related to vaccine injections. None led to premature study discontinuation. No death was reported during the study period.


The study results demonstrate that concomitant administration of the inactivated hepatitis A study vaccine did not interfere with the immunogenicity of the MMR vaccine currently used in the NIP of Turkey and did not diminish the seroprotective response to the hepatitis A vaccine. Descriptive comparisons of seroprotection rates one month after primary vaccination revealed no significant difference in the antibody responses elicited by concomitant versus non-concomitant administration of hepatitis A and MMR vaccines.

Although the hepatitis A post-primary SP rates (≥20 mIU/mL, MEIA assay) were high (93.60% and 92.68%, after separate and concomitant vaccination, respectively), they were lower than the expected 99% seen in previous study results [12]. Although the group difference in SP rates was <1.0%, non-inferiority of concomitant administration was not demonstrated, probably because the SP rates were lower than expected. Non-inferiority of seroprotection (≥20 mIU/mL) against hepatitis A was demonstrated using the more sensitive ECLIA assay, which resulted in SP rates of 100% and 99.39% for separate and concomitant administration, respectively.

The demonstration of a strong immune response to primary vaccination, i.e., a single dose of hepatitis A vaccine is significant in light of the current public health interest in adopting one-dose hepatitis A vaccination in various National Immunization programs [2]. There is evidence for the protective efficacy of a single dose of inactivated hepatitis vaccine from national surveillance in Argentina, where the study vaccine is used in the National Immunization Program, and from recent study reports [13-15]. A single dose may be a useful option for countries where the 2-dose schedule may not be possible, and only one dose can be administered universally or to special target groups. Additional studies of long-term immunogenicity and boosting potential are needed [2].

Few hepatitis A infections have been reported among vaccinated persons, and vaccine-induced antibody levels are generally high. Consequently, a hepatitis A seroprotection threshold following vaccination has not yet been identified. Anti-HAV concentrations of 20 mIU/mL after administration of immunoglobulins are known to protect against hepatitis A [11,16]. However, in vitro studies have shown that depending on the test system used, neutralizing antibodies against hepatitis A may still be present even if the measured concentration is less than 20 mIU/mL [17]. As no absolute protective level has been defined, the LLOQ of the assay used (i.e., detectable hepatitis A antibodies) is often considered to indicate seroprotection [11,18]. The LLOQ of the assay method used in this study was 5 mIU/mL for the MEIA and 3 mIU/mL for the ECLIA assay; thus we have considered both ≥5 and ≥20 mIU/mL as seroprotective.

Quantitation of anti-HAV concentration depends on the assay used, with varying sensitivity resulting from differences in design or reagents that affect the ability to detect low antibody concentrations [17,19,20]. As measured by the MEIA assay in this study, post primary anti-HAV antibody concentrations were lower than expected [12]. Nevertheless, our anti-HAV results are consistent with other reports that found the Elecsys Anti-HAV ECLIA assay to be more sensitive than the AxSYM HAVAB 2.0 MEIA assay [20,21]. In one series, the MEIA assay detected seroconversion in 10% fewer subjects 2 weeks after vaccination than the ECLIA assay, with part of the observed difference thought to have resulted from a differential ability to detect IgM [20]. Such a difference might account for both the relatively low anti-HAV concentrations observed by MEIA assay after primary vaccination in this study as well as increasing concentrations over the 6 months prior to booster administration.

From 58.3% to 62% of participants had anti-HAV concentrations above the lower limit of detection of the MEIA assay used for preenrollment screening. Those antibodies were most likely of maternal origin as the GMC decreased in Group B prior to administration of hepatitis A vaccine on Day 28. Seroepidemiological studies in Turkey have shown persistence of maternal anti-HAV for up to 18 months of age; seropositivity rates of infants at 3, 6, 9, 12, 15, and 18 months of age were 90.5%, 84.4%, 62.6%, 36.1%, 13.6%, and 6.1%, respectively [22]. The presence of maternal antibodies in our study participants did not interfere with priming and boosting with the study hepatitis A vaccine; a result that is consistent with previous reports [22-24]. Anti-HAV concentrations increased strongly in all study groups after the booster injection, suggesting that priming had been successful following both study schedules and regimens.

Seroprotection rates against measles, mumps and rubella and GMCs were low in all groups at enrollment and decreased in Group A until MMR vaccination on Day 28. The seroprotection rates of at least 96.5% in both Group B and Group C indicate that MMR vaccine induced a satisfactory immunological response when administered concomitantly with the hepatitis A vaccine.

The study vaccines were well tolerated, with solicited reactions generally of Grade 1 or 2 intensity and resolved without treatment within a few days. Reactions were less frequently reported after the booster dose than after the first dose. Reactogenicity was slightly greater following concomitant administration, with about 8% more participants reporting a solicited injection site reaction and 10% more reporting a solicited systemic reaction.


The immune response to the hepatitis A vaccine evaluated in this study, as measured by seroprotection rates and GMCs, was not impaired by concomitant administration with MMR vaccine. Noninferiority of concomitant, compared to separate administration of hepatitis A and MMR vaccines was demonstrated for seroprotection rates when measured with a sensitive assay for anti-hepatitis A antibody concentrations. While reactogenicity was slightly increased with concomitant administration of the two study vaccines, the vaccines were well tolerated.


The authors thank the children and their parents for participation, and also Serdar Altinel for his help for running the trial. The authors would also like to thank Clement Weinberger of The Stylus Medical Communication for assistance in the preparation of the manuscript on behalf of Sanofi Pasteur and in accordance with the European Medical Writers Association guidelines.


TP and AR are employees of Sanofi Pasteur. KY, MB and AS have received honoraria from Sanofi Pasteur for conducting this clinical study. This study was conducted with the financial support of Sanofi Pasteur, Lyon, France.


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