The seed and leaf protein content was estimated by adopting the methodology. 50 mg of oven dried black gram seeds and leaves were ground with the addition of 1 ml of 5% trichloroacetic acid. The absorbance was read at 660 nm using spectrophotometer. The total protein content was calculated by comparing the absorbance of each sample with a calibration curve plotted by taking known graded concentration of bovin serum albumin.
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Citation: Akhtar A, Hisamuddin, Abbasi, Sharf R (2013) Study on Black Gram (Vigna mungo L.) Infected with Meloidogyne incognita under the Influence of Pseudomonas fluorescens, Bacillus subtilis and Urea. J Plant Pathol Microb 4: 202.
Last date updated on July, 2014