alexa Method Development and Validation for estimation of Moxifloxacin HCl in tablet dosage form by RP-HPLC method | Open Access Journals
ISSN : 2153-2435
Pharmaceutica Analytica Acta
Like us on:
Make the best use of Scientific Research and information from our 700+ peer reviewed, Open Access Journals that operates with the help of 50,000+ Editorial Board Members and esteemed reviewers and 1000+ Scientific associations in Medical, Clinical, Pharmaceutical, Engineering, Technology and Management Fields.
Meet Inspiring Speakers and Experts at our 3000+ Global Conferenceseries Events with over 600+ Conferences, 1200+ Symposiums and 1200+ Workshops on
Medical, Pharma, Engineering, Science, Technology and Business

Method Development and Validation for estimation of Moxifloxacin HCl in tablet dosage form by RP-HPLC method

P Rama Subbaiah, M V Kumudhavalli*, C Saravanan, M Kumar and R Margret Chandira
Department of Pharmaceutical Analysis, Vinayaka Mission’s College of Pharmacy, Vinayaka Missions University, Salem-636008, Tamilnadu, India
Corresponding Author : Dr. M.V.Kumudhavalli
3D/58, Babu Nagar
Salem–636001, Tamilnadu, India
Tel: 919942574759
Received August 30, 2010; Accepted September 27, 2010 Published September 30, 2010
Citation:Subbaiah PR, Kumudhavalli MV, Saravanan C, Kumar M, Chandira RM (2010) Method Development and Validation for estimation of Moxifloxacin HCl in tablet dosage form by RP-HPLC method. Pharm Anal Acta 1:109.doi:10.4172/2153-2435.1000109
Copyright: © 2010 Subbaiah PR, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Related article at
DownloadPubmed DownloadScholar Google

Visit for more related articles at Pharmaceutica Analytica Acta


A rapid, sensitive and specific RP-HPLC method involving UV detection was developed and validated for determination and quantification of Moxifloxacin HCl in tablet dosage form. Chromatography was carried out on a pre-packed Luna C-18, 5μ (250 x 4.6) mm column using filtered and degassed mixture of Buffer: Methanol (55:45) as mobile phase at a flow rate of 1.0 ml/min and effluent was monitored at 293 nm. The pH of the mobile phase was adjusted with acetic acid to 6.3±0.4. The method was validated in terms of linearity, precision, accuracy, and specificity, limit of quantification and limit of detection. The assay was linear over the concentration range of 20 mcg-60 mcg/ ml. Accuracy of the method was determined through recovery studies by adding known quantities of standard drug to the pre analysed test solution and was found to be 99.3 %-100.2 % within precision RSD of 0.58 for Moxifloxacin HCl. The system suitability parameters such as theoretical plates, retention time factor and tailing factor were found to be 7968, 5.855 and 1.207 respectively. The method does require only 10 min as run time for analysis which proves the adoptability of the method for the routine quality control of the drug.

Moxifloxacin HCl; RP-HPLC; Estimation; Validation
Moxifloxacin HCl is chemically 1-cyclopropyl-7-((s,s)-2,8- diazabicyclo(4.3.0)non-8-yl)-6- fluoro-8-methoxy-1,4-dihydro-4-oxo-3 quinoline carboxylic acid. Moxifloxacin is an antibiotic used to treat respiratory infections, including acute sinusitis, acute exacerbations of chronic bronchitis, and community-acquired pneumonia, as well as dermatological infections, as a second-line agent in tuberculosis. The literature survey [1-7] reveals that there is some HPLC methods have been reported. In this paper we describe a simple, inexpensive, sensitive and validated HPLC [8-12] method for the determination of Moxifloxacin HCl in bulk and pharmaceutical formulation.
Working standard of Moxifloxacin HCl was obtained from well reputed research laboratories. HPLC grade Methanol, Merck grade Orthophosphoric acid and Triethylamine and Milli-Q water were procured from the market. The separation was carried out on isocratic HPLC system with pre-packed Luna C-18, 5µ (250 x 4.6) mm column using filtered and degassed mixture of Buffer: Methanol (55:45) as mobile phase.
Buffer Preparation
Transferred 2 ml of orthophosphoric acid into 1000 ml of water and the PH were adjusted to 2.5 with triethylamine, filtered through 0.45µm nylon membrane filter and degassed.
Mobile phase
Buffer and methanol were mixed in the ratio of 55:45 and sonicated to degas.
Standard preparation
Accurately Weighed and transferred Moxifloxacin HCl equivalent to 20 mg of Moxifloxacin Working Standard into a 50 ml clean dry volumetric flask, and 30 ml of mobile phase was added, sonicated for 5 minutes, and diluted to volume with mobile phase. Further diluted 5 ml to 50 ml with mobile phase.
Flow rate 1.0 ml/min; detection wavelength 293 nm; injection volume 10µl; column used Luna C18 (5µm, 250x4.6 mm); column temperature: 25oC; mobile phase: Buffer: Methanol (55:45).
Working standard of various concentrations was prepared by taking aliquots of standard solution and diluted to get required concentration for calibration plot and which was injected.
Sample preparation
Weighed and powered 20 tablets. Transferred the powder equivalent to 400 mg of Moxifloxacin into 100 ml of clean, dry, volumetric flask and, to this added 70 ml of mobile phase and sonicated for about 15 minutes, further made up the volume with mobile phase and then filtered through 0.45 micron filter. Further diluted 1 ml of the filtrate to 100 ml with mobile phase. 10µl of the standard preparation and assay preparation were separately injected and chromatographed.
Linearity [13-18] was demonstrated by analysing six different concentrations of active compound. Peak areas were recorded for all the peaks and calibration plot was constructed by plotting peak area vs. concentrations of Moxifloxacin HCl which were found to be linear in the range of 20 mcg/ml-60 mcg/ml. Coefficient of correlation was 0.9990 (Figure 1). Accuracy [13-18] was done by recovery study using standard addition method, known amount of standard Moxifloxacin HCl in to pre-analysed samples and subjected to proposed HPLC method. The results of recovery studies are shown in (Table 1).
To demonstrate agreement among results, a series of measurements are done with Moxifloxacin HCl six replicate injections of the specific standard at various time intervals on the same day were injected into the chromatograph and the value of % RSD was found to be 0.58. In inter-day precision [13-18] same standard was injected on different days and the found % RSD was found to be 0.95 %. The results were showed in the (Table 2).
The regression value was found to be 0.9990 for Moxifloxacin HCl, which shows the response is linear from 20µg/ml to 60µg/ ml. Selectivity experiment showed that there is no interference or overlapping of the peaks either due to excipients or diluents with the main peak of Moxifloxacin HCl. The percentage RSD for precision is < 2 which confirms that method is sufficiently precise and the total run time required for the method is only 10 min for eluting Moxifloxacin HCl. The proposed method is simple, fast, accurate, and precise and can be used for routine analysis in quality control of Moxifloxacin HCl.

Tables and Figures at a glance

Table icon Table icon
Table 1 Table 2


Figures at a glance

Figure Figure
Figure 1 Figure 2
Select your language of interest to view the total content in your interested language
Post your comment

Share This Article

Recommended Conferences

Article Usage

  • Total views: 11680
  • [From(publication date):
    November-2010 - Apr 24, 2017]
  • Breakdown by view type
  • HTML page views : 7870
  • PDF downloads :3810

Post your comment

captcha   Reload  Can't read the image? click here to refresh

OMICS International Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

1-702-714-7001Extn: 9037

Business & Management Journals


1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

1-702-714-7001 Extn: 9042

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version