The samples were then cooled to room temperature and washed with distilled water. The endogenous peroxidases were blocked with 0.9% hydrogen peroxide, and the samples were again washed with distilled water and phosphate-buffered saline solution (PBS, pH 7.4). The primary antibodies were incubated for 30 minutes for Survivin (Clone 12C4, 1 : 100, Dako Corp, Carpinteria, CA, USA), Bcl-2 (Clone 124, 1 : 50, Dako Corp, Carpinteria, CA, USA), Bax (Polyclonal, 1 : 150, Dako Corp, Carpinteria, CA, USA) and Ki-67 (Clone MIB-1, 1 : 50, Dako Corp, Carpinteria, CA, USA). Subsequently, the sections were incubated with the biotinylated anti-mouse/anti-rabbit secondary antibody and streptavidin/peroxidase complex (LSA-B + labeled streptavidin-biotin, Dako Corp., Carpinteria, CA, USA) for 30 minutes each. The reaction products were displayed with 3,3′-diaminobenzidine-H2O2 substrate (Dako Corp, Carpinteria, CA, USA).
The case records and surgical biopsy reports of all diagnosed cases of ameloblastoma archived from January 2010 to December 2012 were retrieved from the Outpatient Department. Histopathology will show cells that have the tendency to move the nucleus away from the basement membrane. This process is referred to as "Reverse Polarization". While chemotherapy, radiation therapy, curettage and liquid nitrogen have been effective in some cases of ameloblastoma, surgical resection or enucleation remains the most definitive treatment for this condition.
There is evidence that suppression of matrix metalloproteinase-2 may inhibit the local invasiveness of ameloblastoma, however, this was only demonstrated in vitro. There is also some research suggesting that α5β1 integrin may participate in the local invasiveness of ameloblastomas.