Sappinia is a free-living ameba, or amoeba* (a single-celled living organism), found in the environment. There are two known species of Sappinia: Sappinia diploidea and Sappinia pedata. This ameba causes amebic encephalitis, which is an infection of the brain. Worldwide, only one case of amebic encephalitis due to Sappinia infection has been reported.
The clinical samples of cerebrospinal fluid and brain tissue obtained from patients with suspected cases of free-living amoeba infection as well as DNA from other pathogens that cause similar infections (see Methods for details) also tested negative in this TaqMan assay.species TaqMan assay produced positive results for DNA extracted from the 2 cultured strains, with a detection limit of approximately 1.4 cells/mL. The assay did not detect DNA from cultures of or . Furthermore, a collection of