alexa Next-Generation Sequencers: What Can We Learn? | OMICS International
ISSN: 1948-593X
Journal of Bioanalysis & Biomedicine
Like us on:
Make the best use of Scientific Research and information from our 700+ peer reviewed, Open Access Journals that operates with the help of 50,000+ Editorial Board Members and esteemed reviewers and 1000+ Scientific associations in Medical, Clinical, Pharmaceutical, Engineering, Technology and Management Fields.
Meet Inspiring Speakers and Experts at our 3000+ Global Conferenceseries Events with over 600+ Conferences, 1200+ Symposiums and 1200+ Workshops on
Medical, Pharma, Engineering, Science, Technology and Business

Next-Generation Sequencers: What Can We Learn?

Tatsuya Mimura1,2*, Hidetaka Noma3, Hideharu Funatsu4 and Satoru Yamagami1,2

1Department of Ophthalmology, Tokyo Women’s Medical University Medical Center East, Tokyo 116-8567, Japan

2Department of Ophthalmology, University of Tokyo Graduate School of Medicine, Tokyo 113-8655, Japan

3Department of Ophthalmology, Hachioji Medical Center, Tokyo Medical University, Tokyo 193-0998, Japan

4Department of Ophthalmology, Yachiyo Medical Center, Tokyo Women’s Medical University, Yachiyo, Chiba 276-8524, Japan

*Corresponding Author:
Tatsuya Mimura, MD, Ph.D
Department of Ophthalmology
Tokyo Women’s Medical University Medical Center East
2-1-10 Nishiogu, Arakawaku, Tokyo116-8567, Japan
Tel: +81-3-3810-1111 (Ex7765)
Fax: +81-3-3894-0282
E-mail: [email protected]

Received Date: May 02, 2013; Accepted Date: May 03, 2013; Published Date: May 06, 2013

Citation: Mimura T, Noma H, Funatsu H, Yamagami S (2013) Next-Generation Sequencers: What Can We Learn? J Bioanal Biomed 5:e113. doi:10.4172/1948-593X.1000e113

Copyright: © 2013 Mimura T, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Visit for more related articles at Journal of Bioanalysis & Biomedicine

The epigenome is defined as DNA methylation of all genes that influences expression without alteration of the DNA sequence itself [1]. Epigenetic mechanisms include DNA methylation and histone modification [2], as well as nucleosome repositioning [3-5]. The nextgeneration sequencers (NGS) provide an attractive opportunity for studying DNA methylation patterns.

The NGS were released several years ago, but the high cost limited their popularity in those days. More recently, extensive sequence analysis performed by the NGS has provided rapid and low-cost DNA sequencing. These machines are becoming increasingly available and are currently used at many laboratories. NGS will ultimately contribute to elucidation of the genomic mechanisms responsible for various diseases.

What we can learn from the NGS? The technology provides an efficient method for epigenome-wide analysis, allowing easy detection of disease-related genes and genetic variations. This new approach can be applied to obtain high-quality sequence data by using the genomic DNA isolated from a single cell. For example, NGS provided the substantial breakthrough that led to the first practical applications in analysis of cancer genomics. Scientists are currently sequencing genomic DNA extracted from various cancers and comparing the whole cancer genome with the normal genome. We can identify functionally important cancer-related gene, which act to transform normal cells into tumor cells, by comprehensive comparison of various cancer genomes with the normal genome. Furthermore, recent advances in cancer research based on NGS technology have allowed scientists to identify cancer-specific alleles that are responsible for malignant behavior and can be used to predict the responsiveness to treatment and prognosis of cancer. The second impact of this evolving technology is identification of individual susceptibility genes for complex diseases. Analysis of single nucleotide polymorphisms (SNPs) has previously been used in genome-wide association studies (GWAS) to identify genetic variants that influence susceptibility to common diseases or complex diseases. However, analysis of SNPs takes a long time. Exon sequencing with NGS seems to be the most effective strategy to reduce costs and save time, while improving both quality and productivity. The third important advance is that NGS protocols have recently been extended to analyze DNA methylation. Analysis of DNA methylation relies on bisulfite conversion of DNA. We can analyze the genomewide DNA methylation profile of people with age-related diseases and can compare the methylation profile between cells from diseased organ and cells from the unaffected organs of healthy people.

However, use of NGS has some problems. One of the important issues raised by NGS technology is how to analyze enormous amounts of sequence data correctly within a limited period. The second issue is how to select specific genes from the huge number of candidate signals identified by NGS. The third issue is the ethical, legal, and social consequences of mapping and sequencing the human genome. Handling genomic data requires careful attention to the protection of personal information. If these issues can be solved, NGS technology could become a valuable tool for use in the identification of susceptibility genes for complex diseases.

References

Select your language of interest to view the total content in your interested language
Post your comment

Share This Article

Recommended Conferences

Article Usage

  • Total views: 11525
  • [From(publication date):
    June-2013 - Dec 11, 2017]
  • Breakdown by view type
  • HTML page views : 7770
  • PDF downloads : 3755
 

Post your comment

captcha   Reload  Can't read the image? click here to refresh

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version